Protein purification Study guides, Revision notes & Summaries

Summary NUTR 405 Complete A+ Study guide_ Fall 2024 Proximate Analysis Lecture 1. List the five proximate components, their importance to food analysts, and the various methods/apparatus that each can be determined. **just the methods/apparatus that we covered in lab-not the numerous other methods of analysis that are covered in the text book Proximate Analysis: quantitative analysis of any food to determine the percentage of its components o Determine moisture, ash, fat, protein and carbo...

ACS BIOCHEMISTRY Solved Exam |100% Pass| Henderson-Hasselbach Equation *Ans* pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis *Ans* Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) *Ans* Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography *Ans* Separates sample based on ...

_______ use an internal RNA primer so that the end of the eukaryotic chromosome can be replicated. a. Primases b. RNA polymerases c. Telomerases d. DNA polymerases e. Helicases c. Telomerases A transmembrane sensor kinase protein senses an environmental condition outside __________ bacteria or in the periplasm of a/an __________ bacteria. a. Gram-positive; Gram-negative b. Gram-negative; Gram-positive c. prokaryotic; eukaryotic d. prokaryotic; archaean e. Gram-positive; aci...

Which of these changes to protein purification using an AKTA would most likely be classified as minor change? The flow rate was increased 25% higher. The buffer that is used in the mobile phase was replaced with a different weak acid The set number of times the column can be washed and reused is changed from 20 to 25 reuses. The tempeature setting for the column was decrease 25% from the earlier settings. The detector was replaced with a newer model that is more sensitive in detecting low...

ALL CIDESCO QUESTIONS EXAM 772 QUESTIONS AND CORRECT ANSWERS(VERIFIED ANSWERS)|AGRADE In an isometric muscle exercise the joints are - Answer -Not moved in spite of tightening and relaxing the muscles A figure assesement should be carried out - Answer -Every 6 treatments An abnormailty of the vertebral column which gives a humped back appearance is - Answer -Kyphosis The use of a plumb line in a figure correction is important to - Answer -Assess postural defects Cellulite is cause...

Everyone who may have contact with biohazard items must be educated about dangers associated with those items True "-Pexy" is Surgical fixation Infectious is medical Waste is regulated by the EPA True "Dermopathy" is a (an) Skin disease The bacteria reproductive process that takes place when a mother cell divides into a daughter cell is called Binary fission Autoclave sterilizer is a ____________ class of device Class 1 Promotes healing prevent...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 In a phenol-chloroform extraction, DNA will be in the aqueous phase - answerWhich is true regarding DNA purification? (a) In a phenol-chloroform extraction, DNA will be in the aqueous phase (b) the most common way to purify plasmid DNA is a cesium chloride gradient (c) the best way to purify DNA is via anion exchange chromatography (d) DNA w/ an A260:A280 of 1.25 is pure (e) a miniprep will produce -1 mg of DNA PolyTs - answe...

QBM Final Exam Questions And Answers With Complete Solutions 2024 What is recombinant DNA technology? - answerThe process of taking a gene from one source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and inserting that DNA into bacteria for further study/manipulation Probes - answerSmall pieces of DNA can be used to determine the presence or absence of an allele, which can indicate a predisposition to a specific disease Microarrays - answerAdvance pro...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 What is recombinant DNA technology? - answerThe process of taking a gene from one source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and inserting that DNA into bacteria for further study/manipulation Probes - answerSmall pieces of DNA can be used to determine the presence or absence of an allele, which can indicate a predisposition to a specific disease Microarrays - answerAdvance probe-b...

Chapter 3—Thermodynamics of Biological Systems MULTIPLE CHOICE 1. Thermodynamics does NOT: a. describe the flow and interchange of heat, energy, and matter. b. allow the determination of whether a reaction is spontaneous. c. provide information on the rate of a reaction. d. consider heat flow and entropy production. e. consider the effect of concentration on net free energy change of a reaction. ANS: C PTS: 1 2. Living systems are: a. closed systems exchanging only energy with the s...