Protein purification Study guides, Revision notes & Summaries
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ACS BIOCHEMISTRY EXAM COMPLETE SOLUTION UPDATED 2024
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Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) 
FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 
Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. 
Ion-Exch...
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ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT
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ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT 
 
Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) 
 
FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
 
Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 
 
Size-Exc...
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Albert's Chapter 8 Exam 115 Questions with Verified Answers,100% CORRECT
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Albert's Chapter 8 Exam 115 Questions with Verified Answers 
 
WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter 
 
How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type 
 
How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...
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QBM Exam 1 Questions And Answers With Complete Solutions 2024
- Exam (elaborations) • 77 pages • 2024
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QBM Exam 1 Questions And Answers With 
Complete Solutions 2024 
Chapter 1: Overview of the Molecular Biology 
Geber 1800s: He developed ____ that make ways for molecular biology. 
From 1700s - 1900s there are huge advancements in ___ 
(start studying small molecules) 
1930s- Understanding of ________ happen 
1938s - "Molecular Biology" was written by ______. 
(First time the word molecular biology actually happen) 
1944s - _____ shows DNA was the basis for genes 
1953 - ____ and ____ determine...
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WGU C001 BIOCHEMISTRY STUDY GUIDE QUESTIONS WITH VERIFIED ANSWERS
- Exam (elaborations) • 18 pages • 2023
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WGU C001 BIOCHEMISTRY STUDY GUIDE QUESTIONS WITH VERIFIED ANSWERS 
 
 
Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) 
 
FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chainto a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
 
Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 
 
Size-Exclusion Chromatog...
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BSC 2010C Chapter 5: Proteins: Primary Structure Questions & Answers From The Real Exam |Rated A+.
- Exam (elaborations) • 11 pages • 2024
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Matching 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid 
F) negative charge 
G) nucleases 
H) chromophore 
I) foaming 
J) high level expression 
K) 2-mercaptoethanol 
L) positive charge 
M) cation exchange 
N) pI 
O) chymotrypsin 
P) C-terminal amino acid 
Q Sodium dodecyl sulfate 
1. One of the reasons the primary structure is important for a protein is that it determines the 
______ the molecule adopts...
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Biological Chemistry of Organic Cofactors - BC2 Questions With Complete Solutions
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What is the proteome of the cell? correct answer: the full complex of proteins that can be produced using genetic material and post translational modifications 
 
What are some example of post translational modifications? correct answer: phosphorylation (signalling) 
GPI anchor (PM proteins) 
O-glycans (nuclear and cytosolic proteins) 
polyubiquitination (can induce protein degredation) 
 
How can you watch the purification of recombinant poly-His tagged GFP? correct answer: use Ni2+ immobili...
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QBM Exam 1 Questions And Answers With Complete Solutions 2024
- Exam (elaborations) • 37 pages • 2024
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QBM Exam 1 Questions And Answers With 
Complete Solutions 2024 
What is recombinant DNA technology? - answerThe process of taking a gene from one 
source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and 
inserting that DNA into bacteria for further study/manipulation 
Probes - answerSmall pieces of DNA can be used to determine the presence or absence of 
an allele, which can indicate a predisposition to a specific disease 
Microarrays - answerAdvance probe-b...
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QBM Exam 1 Questions And Answers With Complete Solutions 2024
- Exam (elaborations) • 77 pages • 2024
- Available in package deal
-
- £12.46
- + learn more
QBM Exam 1 Questions And Answers With 
Complete Solutions 2024 
Chapter 1: Overview of the Molecular Biology 
Geber 1800s: He developed ____ that make ways for molecular biology. 
From 1700s - 1900s there are huge advancements in ___ 
(start studying small molecules) 
1930s- Understanding of ________ happen 
1938s - "Molecular Biology" was written by ______. 
(First time the word molecular biology actually happen) 
1944s - _____ shows DNA was the basis for genes 
1953 - ____ and ____ determine...
-
QBM Final Exam Questions And Answers With Complete Solutions 2024
- Exam (elaborations) • 114 pages • 2024
- Available in package deal
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- £14.07
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QBM Final Exam Questions And Answers 
With Complete Solutions 2024 
What is recombinant DNA technology? - answerThe process of taking a gene from one 
source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and 
inserting that DNA into bacteria for further study/manipulation 
Probes - answerSmall pieces of DNA can be used to determine the presence or absence of 
an allele, which can indicate a predisposition to a specific disease 
Microarrays - answerAdvance pro...
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