Km and vmax Study guides, Revision notes & Summaries

BIOCHEMISTRY ACS EXAM QUESTIONS WITH 100% CORRECT ANSWERS Unfolds proteins and gives them uniform negative charge. - answerSDS Reacts with the N-terminus of the protein to produce a 2,4-dinitrophenol derivative that labels the first residue. Can repeat hydrolysis to determine sequential amino acids - answerFDNB Reduces disulfide bonds. - answerDTT Adds carboxymethyl group on free -SH groups. Blocks disulfide bonding. - answerIodoacetate Bonds between two -SH groups that form between 2°...

Membrane Transport What is the major difference between V-ATPase and F-ATPase? - ANSWERSV-ATPase uses ATP to pump hydrogen against the concentration gradient; F-ATPase uses hydrogen concentration gradient to make ATP Why can't H3O+ flow through an aquaporin channel? - ANSWERSH3O+ size and charge; positively charged Asn repel H3O+ How would F-ATPase be affected with H3O+ could flow through aquaporins? - ANSWERSChange H+ gradient, affecting function of F-ATPase and production of ATP How...

Measurement of alkaline phosphate in plasma (blood) can be used for diagnosis of bone and liver diseases. True or False correct answers True Which pipette is most appropriate for measuring 0.1mL? a) P20 b) P200 c) P1000 d) P5000 correct answers b) P200 Which pipette is most appropriate for measuring 0.1mL? a) P20 b) P200 c) P1000 d) P5000 correct answers b) P200 Why is a buffer used in the assays? a) The buffer provides substrate from the reaction. b) The buffer cause...

UNIT 1 UNE CHEM 1005 EXAM QUESTIONS AND ANSWERS Hemoglobin binds oxygen with a greater affinity in the: R state Hemoglobin bound to heme is termed a holoprotein. The heme or porphoryin ring is required for oxygen binding and it is termed a: prosthetic group Alpha helices and beta sheets are primarily stabilized by: H bonding Ligases are a class of enzymes characterized by their ability to: synthesize bonds between carbon atoms and an additional atom that is involved in the cleavage of ...

VET272 - Prac Exam 2023/2024 questions and answers complete Measurement of alkaline phosphate in plasma (blood) can be used for diagnosis of bone and liver diseases. True or False True Which pipette is most appropriate for measuring 0.1mL? a) P20 b) P200 c) P1000 d) P5000 b) P200 Which pipette is most appropriate for measuring 0.1mL? a) P20 b) P200 c) P1000 d) P5000 b) P200 Why is a buffer used in the assays? a) The buffer provides substrate from the reaction. b) The ...

Biochemistry Final Exam Practice 63 Questions with Verified Answers The central dogma of molecular biology teaches us that: a. RNA replicates itself and DNA is translated into protein b. RNA is typically the genetic material c. RNA contains information, which is translated into amino acid sequences d. Proteins are not needed to replicate DNA or transcribe RNA e. RNA is made of the same monomers as DNA - CORRECT ANSWER c. RNA contains information, which is translated into amino acid sequ...

UNIT 1 UNE CHEM 1005 EXAM QUESTIONS AND ANSWERS Hemoglobin binds oxygen with a greater affinity in the: R state Hemoglobin bound to heme is termed a holoprotein. The heme or porphoryin ring is required for oxygen binding and it is termed a: prosthetic group Alpha helices and beta sheets are primarily stabilized by: H bonding Ligases are a class of enzymes characterized by their ability to: synthesize bonds between carbon atoms and an additional atom that is involved in the cleavage of ...

VET272 - Prac Exam 2023/2024 questions and answers complete Measurement of alkaline phosphate in plasma (blood) can be used for diagnosis of bone and liver diseases. True or False True Which pipette is most appropriate for measuring 0.1mL? a) P20 b) P200 c) P1000 d) P5000 b) P200 Which pipette is most appropriate for measuring 0.1mL? a) P20 b) P200 c) P1000 d) P5000 b) P200 Why is a buffer used in the assays? a) The buffer provides substrate from the reaction. b) The ...

1. A weak acid, HA, has a pKa of 4.11. a) At pH 7.11, what will the ratio of conjugate base to acid be? (Henderson-Hasselbach equation: pH=pKa + log [A-]/[HA]) b) Based on answer to "a," is there more HA or A- at that pH? c) At pH 2.11, what will the ratio of conjugate base to acid be? d) Based on answer to "c," is there more HA or A- at that pH? e) At pH 4.11, what will the ratio of conjugate base to acid be? f) Fill in the blank: if pH is greater than the pKa of a weak acid, there w...

How do most enzymes reduce the activation energy needed to move a reaction forward? Providing an active site most complementary the transition state We have an expert-written solution to this problem! An enzyme has a mutation within the substrate binding of the coenzyme needed for covalent catalysis. Which of the following is likely to result as a consequence of this mutation? The enzyme will not be able to form the transition state complex Hemoglobin has the ability to display ...