Gene cloning Study guides, Revision notes & Summaries

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Application of Biotechnology for Plant Breeding & Genetics 2 o Gene cloning o Plant transformation o Molecular analysis o Genomic tools o Marker assisted breeding o Course orientation o Why alter plants o Why use biotechnology o Biotechnology strategies Outline 3 o Course orientation o Why alter plants o Why use biotechnology o Biotechnology strategies Outline 5 Dr. Guo-qing Song Associate Professor, Associate Director of PBROC Department of Horticulture A336, PSS Bldg P...

GCD Exam 3 Questions with 100% Pass Which of the following may be used as a vector in a gene-cloning experiment? a) mRNA b) Plasmid c) Virus d) c & d - Answer-d The restriction enzymes used in gene-cloning experiments ___________, which generates sticky ends that can _________. a. cut the DNA, enter bacterial cells b. cut the DNA, hydrogen bond with complementary sticky ends c. methylate DNA, enter bacterial cells d. methylate DNA, hydrogen bond with complementary sticky ends - Answe...

IB Biology Unit 1 Latest Update Graded A+ Cell theory 3 main principles All organisms are composed of one or more cells cells are the smallest units of life all cells come from pre-existing cells 7 functions of life (MR H GREN) metabolism reproduction homeostasis growth excretion response nutrition Paramecium unicellular membrane of the kingdom known as Protista. Look at powerpoint on isa247 to see how this organism displays the functions of life. chlorella single-cell...

Primavera Biology B Unit 3: Genomics (2023/2024) Already Graded A CHECKPOINT 12 Genes located close together on a chromosome tend to be inherited together. What is this called? genetic linkage Which of the following genes are linked in pea plants? flower color and pollen-grain shape A Punnett square incorrectly predicts the outcome of a dihybrid cross. There are only two phenotypes in the F2 offspring instead of four. Why? The genes are completely linked. A Punnett square predicts that the...

MCB Exam 3 Questions & Answers 2023/2024 What is meant by recombinant DNA? - ANSWER-DNA w/fragments from 2+ sources Name the tools used to make a recombinant DNA. (mechanisms of gene cloning) - ANSWER-1) Isolate plasmid DNA from bacterial cell 2) Isolate DNA of interest 3) Insert gene of interest into plasmid DNA 4) Insert now recombinant DNA into bacterium 5) Culture bacteria to make copies Restriction enzymes - ANSWER-endonuclease that cuts DNA at specific sites Cloning vecto...

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

BIO 1A03 Midterm 2 Questions and Answers Graded A Prokaryotic growth and cell division -nutrient-rich environment -favorable temperatures Housekeeping genes -genes required at all times for normal function -all ways transcribed and translated -allow constant maintance for general cellular activity -e.g. structural protein of a cell, DNA and RNA polymerase, genes coding for ribosomal proteins -constitutive Regulated genes genes that can be turned on and off at a needed basis Francois Jacob and...

NCHSE CERTIFICATION REVIEW EXAM REVIEW QUESTIONS AND ANSWERS, RATED A+ genetic testing - -the sequencing of human DNA in order to discover genetic differences, anomalies, or mutations that may prove pathological. gene therapy - -the transplantation of normal genes into cells in place of missing or defective ones in order to correct genetic disorders. stem cell research - -biomedical therapy that focuses on unspecialized human cells that are capable of renewing themselves through cell ...

NCHSE CERTIFICATION REVIEW QUESTIONS AND ANSWERS 100% SOLVED genetic testing ️️the sequencing of human DNA in order to discover genetic differences, anomalies, or mutations that may prove pathological. gene therapy ️️the transplantation of normal genes into cells in place of missing or defective ones in order to correct genetic disorders. stem cell research ️️biomedical therapy that focuses on unspecialized human cells that are capable of renewing themselves through cell ...