Sds page - Study guides, Revision notes & Summaries

Lead Duties: what is your number one priority and name the two categories - Answer- number one priority is to be a role model for the instructors on your team. Two categories are administrative and clinical duties Lead duties: Administrative duties (name and describe all 5) - Answer- 1) upkeep all program materials including the log box and all documents contained in the log box: -service verification forms - program plans (program title, SDs, responses and targets all current and dated) ...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample...

NATEF certifies automotive: A dealerships. B independent garages. C technicians. D training programs. D All of these are ASE certifications except: A automatic transmission/transaxle. B engine repair. C service and maintenance. D suspension and steering. C Which part is most likely the problem if a light does not come on during an instrument panel bulb check? A bulb B fuse C sender D switch A What organization certifies automotive technicians? A ASE B AYES ...

BACE Quizlet Questions and Answers Graded A+ Plant cell walls are made of cellulose plants have ___________ _____________ in the roots which spread where and do what? growth hormone, spread to the tips and create differentiation Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:10 / 0:15 Full screen Brainpower Read More agarose is made from and used in polysaccharides from seaweed, gel electrophoresis. agar is made from and used for. br...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exch...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - the characteristic pH at which the net electric charge is zero SDS - Sodium Dodecyl Sulfate (chap ...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

BACE test Questions and Answers A technician is assigned the task of inserting a gene, lanA, into a plasmid to ultimately be expressed as a therapeutic protein in E. coli. Label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. Transform E. coli with the plasmid containing lanA. Digest the amplified lanA PCR product and plasmid with appropriate restriction enzymes. Design primers to amplify the lanA sequence using polymerase chai...

What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used in SDS-PAGE to denature proteins so they separat...