Sds page Study guides, Revision notes & Summaries

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

What is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, what is tap water is best used for? - Answer- Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions. What is agar? - Answer- Solid media on which bacteria grow. What part of an amino acid differs from one to the other? - Answer- R g...

Biochemistry Exam #2 (Module 3) questions and answers 100% verified. (Exam question 21) Dialysis - correct ates small molecules from large molecules (Exam question 30) When a protein is 'salted-in', it precipitates and is removed form a solution. - correct answers.False When we purify protein, we are going against the natural tendency toward disorder (we are fighting against entropy). - correct answers.True When centrifuging sub-cellular structures, what organ...

What is an example of a product easily found in nature? Antibiotic In a biotechnology lab, what is tap water is best used for? Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? mRNA How do DNA molecules vary from one species to another? Number of genes and non-coding regions. What is agar? Solid media on which bacteria grow. What part of an amino acid differs from one to the other? R group How can colorless prot...

FL 5 AAMC MCAT EXAM 2024 What is the chemical structure of a component found in four of the five cofactors used by Na+- NQR? -Answer-says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has an uniquinone AND HERE IS IS MENTIONED T...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

Biotechnician Assistant Credentialing Practice Exam Latest Update Graded A After performing the PCR reaction with the primers she designed to amplify lanA, the same technician found that her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 kilobase pairs. What is the most likely reason there is an unexpected band in her PCR reaction? She used an annealing temperature that was too low In a biotechnology lab, tap wate...

AAMC FL 1 Exam Questions and Answers 2024 If Reaction 2 (Figure 4) is repeated with HCl and the compound shown below, which of the following compounds is NOT a direct product (without rearrangement)? -Answer-Compound A requires a hydride shift to form- therefore not a direct product. NOT an SN2 reaction because of tertiary alcohol- it is SN1 What is the energy of the photons emitted by the LED at a frequency of 610 THz? (Note: h = 6.6 × 10-34 J·s) -Answer-The photon energy is the energy...

ASCP MB TEST VERIFIED SOLUTIONS 100% PASS What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used i...

Gel electrophoresis separates molecules based on what?Changes in size, changes, and shape What side of the electrophoresis chamber does the DNA move and why?Towards the positive side because they are negatively charged What is the SDS-PAGE used today used to separate?denatured proteins During the gel electrophoresis, buffer solution is used to conduct electricity and maintain the pH. T or F?T To prepare .o% agarose gel in 50mL of 1X TAE buffer, how many grams of agarose is needed?.4g