Green fluorescent protein Samenvattingen, Aantekeningen en Examens

Test Bank Essential Cell Biology 5th Edition Alberts Hopkin Table of Contents CHAPTER 1: CELLS: THE FUNDAMENTAL UNITS OF LIFE ................................................................ 2 CHAPTER 2: CHEMICAL COMPONENTS OF CELLS ........................................................................ 21 CHAPTER 3: ENERGY, CATALYSIS, AND BIOSYNTHESIS .............................................................. 48 CHAPTER 4: PROTEIN STRUCTURE AND FUNCTION ......................................

Test Bank for Essential Cell Biology 5th Edition Alberts Hopkin ESSENTIAL CELL BIOLOGY, 5th EDITION CHAPTER 1: CELLS: THE FUNDAMENTAL UNITS OF LIFE Unity and Diversity of Cells 1-1 Living systems are incredibly diverse in size, shape, environment, and behavior. It is estimated that there are between 10 million and 100 million different species. Despite this wide variety of organisms, it remains difficult to define what it means to say something is alive. Which of the following can be desc...

LAB 9 - Green Fluorescent Protein (GFP) PurificationWhat is a protein? Proteins are long chains of amino acids, some of which are hydrophobic Explain the relationship between genes and proteins: Genes contain the genetic code which determine the amino acid composition of a protein. There is a unique gene for each protein within all of the cells of the body Using your own words, describe cloning: The duplication and propagation of a cell or organism. Describe how the bacte...

Test Bank for Essential Cell Biology 5th Edition Alberts Hopkin Test Bank for Essential Cell Biology 5th Edition Alberts Hopkin ESSENTIAL CELL BIOLOGY, 5th EDITION CHAPTER 1: CELLS: THE FUNDAMENTAL UNITS OF LIFE Unity and Diversity of Cells 1-1 Living systems are incredibly diverse in size, shape, environment, and behavior. It is estimated that there are between 10 million and 100 million different species. Despite this wide variety of organisms, it remains difficult to define what it m...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...

Light microscopy CORRECT ANSWER - Uses visible light to examine microscopic-sized objects that cannot be seen by the naked eye. - Allows finer detail than the naked eye. Electron microscopy CORRECT ANSWER - Visualize objects much smaller than those resolved optically. - Use accelerated electrons instead of light photons. - Samples are fixed with chemicals to cross-link molecules to preserve their structure. Fluorescent stains CORRECT ANSWER - Used with light microscopy. - Fluorescence ...

Principles of immunofluorescence staining we want to identify 2 dif proteins, how do we do it? *Make sure that ........ (2 essential parts to note) - First we must generate primary antibodies that recognize/bind our target protein from 2 DIFFERENT species animals. then, use secondary antibody (tagged with color) from a 3rd animal that recognizes/binds the primary but is a dif color than the other secondary antibody *primary antibodies must be from different species *secondary anti...

beta-oxidation correct answer: enzymes repeatedly split off pairs of they hydrogenated carbon atoms that make up a fatty acid and join each pair to coenzyme A to form acetyl-CoA Chloroplasts correct answer: light harvesting structures found in photosynthetic eukaryotes; synthesize a few polypeptides compare and contrast archaeal flagella with bacterial flagella correct answer: archaeal are about half as thick as bacteria; not hollow; lack a central channel; flow together; powered by ATP...

CMM 577 EXAM 2 2024 WITH 100% CORRECT ANSWERSWhat are the three different cytoskeletal elements? Describe them. - correct answer Actin: Responsible for cell protrusions Concentrated at the cell cortex Organize into bundles/networks Microtubules: Long, larger, Made of tubulin Give rise to cilia, mitotic spindles and interphase array Intermediate Filaments: Ropelike fibers made of IF proteins Mechanical strength and support for the entire cell and nuclear lamina Describe the classifi...

Molecular Cell Biology Final EXAM QUESTIONS AND CORRECT ANSWERS(VERIFIED ANSWERS)|AGRADE Separation of most blood cells is difficult, if not impossible, to achieve because they have similar properties and/or densities. What procedure is used to separate T-cells of the immune system from the many other different types of white blood cells or spleen cells? What feature of the T-cell facilitates the isolation protocol? Describe how this procedure is performed. - ANSWER- Flow cytometr...