Crispr cas9 Samenvattingen, Aantekeningen en Examens

Uitwerking van alle examenvragen uit de lijst op canvas gegeven door professor Van der Henst (2021). DISCLAIMER: De lijst met examenvragen varieert ieder jaar een beetje (hangt van gastsprekers af), maar het grootste deel blijft wel altijd hetzelfde. Part 0: Introduction: Prokaryoten Part 1: Prokaryoten: Plasmids and chromosome, selection and counter-selection markers Part 2: Prokaryoten: Gene deletion and cloning techniques Part 3: Promoters, reporter genes and PCR. Part 4: Natural mu...

what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR- Cas9 1. non-homologous end joining (NHEJ) - naturally try to repair - imperfect, creates indels causing frameshift mut...

CMMB 403 final exam Question and answers 2024 verified to pass what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - correct answer - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - correct answer endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - correct an...

In deze samenvatting kan je alle informatie terugvinden die gegeven werd tijdens de hoorcolleges van MGOT deel 1. Hierbij zijn dus de hoorcolleges 1 t/m 9 verwerkt met alle afbeeldingen van de slides op een paar na.

CMMB 403 final exam what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - Answer ️️ -- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - Answer ️️ -endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - Answer ️️ -1. non-homologous end joining (NHEJ) - nat...

CMMB 403 final examwhat does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - correct answer - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - correct answer endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - correct answer 1. non-homologous end joining (NHEJ) - n...

Deze gedetailleerde samenvatting behandelt alles wat aan bod is gekomen tijdens de hoorcolleges, werkcolleges, E-modules, practica en het boek Molecular Biology of the Cell (Alberts 6th edition) gedurende week 7 t/m week 9 van de cursus Genoom aan de Universiteit Utrecht (BMW21614). Hierdoor bevat het alle informatie die nodig is voor het tweede deeltentamen.

CMMB 403 final Exam New Year 2024| Questions and Answers| Certified Quality Graded A+ what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 1. non-homologous end jo...

CRISPR-Cas9 Complete Study Guide Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of ...

CRISPR Cas-9 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Describe i...