Gfp Samenvattingen, Aantekeningen en Examens

Light microscopy CORRECT ANSWER - Uses visible light to examine microscopic-sized objects that cannot be seen by the naked eye. - Allows finer detail than the naked eye. Electron microscopy CORRECT ANSWER - Visualize objects much smaller than those resolved optically. - Use accelerated electrons instead of light photons. - Samples are fixed with chemicals to cross-link molecules to preserve their structure. Fluorescent stains CORRECT ANSWER - Used with light microscopy. - Fluorescence ...

Light Microscopy CORRECT ANSWER Used alongside differential staining i. Gram Staining: identifies gram (+) or gram (-) ii. Fluorescent stains: used to study molecule interaction/aggregation in certain tissue, cell, etc. 1. GFP used to study protein localization Cryo-electron microscopy CORRECT ANSWER Used to visualize objects (e.g. individual atoms) and finer structural cellular details much smaller than those resolved optically Cryo-electron tomography CORRECT ANSWER Creates 3D visua...

PURIFICATOIN BY IMAC OF A SUB-CLONED AND EXPRESSED GFP Aranza Lopez Group B Friday The green fluorescent protein (GFP) of the jellyfish Aequorea Victoria is commonly used as a reporter molecule for localization and expression (Tsien, 1998). To gain experience in current techniques in molecular and medical cell biology, the open reading frame (ORF) of a GFP was sub-cloned into a bacterial expression vector and then purified by immobilized metal affinity chromatography (IMAC). At the e...

BIOD 171 Portage Learning Final EXAM Questions and Answers 100%Correct/Exam 1-7 2023/2024 UpdatePortage Learning BIOD 171 Final EXAM Questions and Answers 100%Correct/Exam 1-7 2023/2024 Update True or False: A virus is considered a microorganism. False. Viruses are not living and as such are not considered microorganisms. Viruses can, however, be classified as microbes, a more general term that includes microorganisms and viruses. 1. What is the smallest biological unit of life? A cell. ...

De inhoud van de theoretische verslag bevat informatie uit deze titels en hoofdstukken. 1.2 Behandelmethode 2.2 Toedieningswegen 2.2.2 Systemische toediening 2.3 Orale toedieningsvorm 2.3.1 Tabletten 2.3.2 Capsules 2.7.1 Zetpillen 3.2 Opname 3.2 Werking van de geneesmiddel 3.3 Omzetting in de lever 3.3.1 Bloedspiegel 3.4 Uitscheiding 5.1 Interactie inleiding 6.1 Contra indicatie 9.1.1 Wat is pijn 9.2.1 Niet opioïden 9.2.2 Opioïden Plus de begrippenlijst e...

De inhoud van de samenvatting bevatten informatie uit deze titels en hoofdstukken + les informatie en informatie van internet. -Onderwerp 11: maag-darmaandoening -Onderwerp 12: anticonceptie -Onderwerp 13: autonome zenuwstelsel -Onderwerp 14: astma en COPD -Onderwerp 15: CVRM + een begrippenlijst die je kunt oefenen voor de toets. Een 8+ voor je toets is gegarandeerd als je de gehele samenvatting geleerd hebt!

CMM 577 EXAM 2 2024 WITH 100% CORRECT ANSWERSWhat are the three different cytoskeletal elements? Describe them. - correct answer Actin: Responsible for cell protrusions Concentrated at the cell cortex Organize into bundles/networks Microtubules: Long, larger, Made of tubulin Give rise to cilia, mitotic spindles and interphase array Intermediate Filaments: Ropelike fibers made of IF proteins Mechanical strength and support for the entire cell and nuclear lamina Describe the classifi...

What is the total volume of reagent in mL? - 0.250mL or 250 microliters pGlo plasmids, when taken up by a bacteria, will code for - a green fluorescence protein GFP Genetic transformation occurs when a cell takes up and expresses a new piece or foreign ____________, often a circular plasmid. - DNA What are 3 real-world links for the study/use of genetic GFP? - study of biological processes, observation of cell movement, use of GFP as a visual marker To genetically transform an entire organ...

Living systems are incredibly diverse in size, shape, environment, and behavior. It is estimated that there are between 10 million and 100 million different species. Despite this wide variety of organisms, it remains difficult to define what it means to say something is alive. Which of the following can be described as the smallest living unit? a) organelle b) DNA c) Cell d) protein - Answer-c The central dogma provides a framework for thinking about how genetic information is copied and ...

De inhoud van de samenvatting bevatten informatie uit deze titels en hoofdstukken + les informatie en informatie van internet: -Toedieningswegen en toedieningsvormen Parenterale toedieningsvormen 2.9 -Middelen bij allergische aandoeningen Inleiding 12.1 Allergie 12.2 Geneesmiddelen bij allergie 12.3 Corticosteroïden 12.3.3 -Beïnvloeding van de weerstand en de afweer Inleiding 20.1 Antigeen, antilichaam en immuniteit 20.2 Vaccin 20.3 Immunoglobuline 20.4 -Infectieziekte ...