Histotechnology Study guides, Revision notes & Summaries

T/F To be characterized as a profession, considerable specialized training must be required. CORRECT ANSWER-T Histology is the study of CORRECT ANSWER-tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. CORRECT ANSWER-T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. CORRECT ANSWER-f T/F The tasks performed by the histotechnologist require patience, mechanical abilit...

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

Block Holder (Chuck) Knife & knife carrier Paul, ratchet feed wheel & adjustment screws CORRECT ANSWER-3 essential parts of microtome Block holder CORRECT ANSWER-Holds the tissue in position Knife and knife carrier CORRECT ANSWER-For actual cutting of tissue sections Pawl, ratchet feed wheel & adjustment screws CORRECT ANSWER-Lines up the tissue block in proper position with knife, adjusting thickness of tissue for successive sections Xylol CORRECT ANSWER-Parts of microtome should ...

HISTOTECHNOLOGY TEST 2 YEAR 3 What stains are used for the detection of acid fast bacteria? - correct answers Kinyoun, Ziel-Neelson, Fite, Auramine-rhodamine fluorescence List the types of pathogenic microorganisms - correct answers Bacteria, Fungi, Viruses, Protozoans, Prions Which microorganism requires extended time in formic acid to be inactivated? - correct answers prions What clues may be present on an H and E to indicate the need for microorganism staining? - correct answers inc...

fixitive used for specimens CORRECT ANSWER-10% neutral buffered Formalin Marking Margins CORRECT ANSWER-- to identify resection margins, if tumour too close to these margins they know they have to go in and cut more out - Guide to embedding What is used to clean grossing station CORRECT ANSWER-phenolic disenfectant or gluteroldehyde how long is specimens stored for CORRECT ANSWER-1-3 months some legal cases kept indefinitly Why is fresh tissue not stored? CORRECT ANSWER-Would decom...

HISTOTECHNOLOGY Art and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence or absence of a disease. Histotechnology Used to view slides not seen by the naked eye microscope Used by pathologist to view slides to identify a disease or process. microscope Used by histotechnologist to view slides FOR QUALITY CONTROL microscope Basic instrument used in microtomy (the process by which processed tissue, most comm...

Histotechnology Final 1st QuarterT/F To be characterized as a profession, considerable specialized training must be required. - correct answers T Histology is the study of - correct answers tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. - correct answers T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. - correct answers f T/F The tasks performed by the histotec...

PART II : HISTOTECHNOLOGYHISTOTECHNOLOGY - correct answers Art and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence of absence of a disease a.) Histology b.) Histopathology c.) Histotechnology d.) Histotechnique AOTA - correct answers INSTRUMENTATION: Laboratory maintain a current file of every piece of equipment in the laboratory including: I. Name, manufacturer, model number, serial number II. Record of preventiv...

Histotechnology Practicum: Test 1Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 2. accession 3. grossing 4. continue fixation 5. dehydration 6. clearing 7. infiltration 8. blocking 9. section 10. staining what is the most i...

Processing includes (no fixation) CORRECT ANSWER dehydration, clearing, and infiltration. Dehydration CORRECT ANSWER the removal of water, a process necessary to prepare the tissue for embedding in a nonaqueous medium such as paraffin, celloidin, and some plastics. Excessive dehydration CORRECT ANSWER when bound water may be removed in a nonadditive reaction. This results in hard and brittle tissue that is difficult to section. Incomplete dehydration CORRECT ANSWER of tissues during pro...