Histotechnology - Study guides, Class notes & Summaries
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PART II : HISTOTECHNOLOGY question n answers graded A+ 2023/2024
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PART II : HISTOTECHNOLOGY 
HISTOTECHNOLOGY - correct answers Art and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence of absence of a disease 
a.) Histology 
b.) Histopathology 
c.) Histotechnology 
d.) Histotechnique 
 
AOTA - correct answers INSTRUMENTATION: 
 
Laboratory maintain a current file of every piece of equipment in the laboratory including: 
 
I. Name, manufacturer, model number, serial number 
II. Record of prevent...
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Carsons Histotechnology with 100% c0rrect answers 2024
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Carsons Mordant 
. substance’s or metals that act as a link between dye and tissue 
 
A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. 
 
 
 
Fixative 
additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble 
 
non additive ...
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HISTOTECHNOLOGY TEST 2 YEAR 3 fully solved graded A+
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HISTOTECHNOLOGY TEST 2 YEAR 3What stains are used for the detection of acid fast bacteria? - correct answers Kinyoun, Ziel-Neelson, Fite, Auramine-rhodamine fluorescence 
 
List the types of pathogenic microorganisms - correct answers Bacteria, Fungi, Viruses, Protozoans, Prions 
 
Which microorganism requires extended time in formic acid to be inactivated? - correct answers prions 
 
What clues may be present on an H and E to indicate the need for microorganism staining? - correct answers incre...
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BOC Histotechnology- Processing correctly answered graded A+ 2023/2024 latest update
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BOC Histotechnology- Processing 
During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. 
 
When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. 
 
Decalcification of small specimens can be achieved by fixation in: - correct answers ZENK...
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Histotechnology Practicum: Test 1 questions & answers 2024
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Histotechnology Practicum: Test 1 
Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy 
 
biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen 
 
What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 
2. accession 
3. grossing 
4. continue fixation 
5. dehydration 
6. clearing 
7. infiltration 
8. blocking 
9. section 
10. staining 
 
what is the most...
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Histotechnology test 2 2024 updated to pass
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Histotechnology test 2 
What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration 
 
Sections will crumble and fall out of block 
 
Shrink the centre of the tissue 
 
Why would a tissue shrink? - correct answers Wax is too hot 
 
What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues 
 
What is the melting point do most paraffins? - correct answers 55-58 de...
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Histotechnology--Nuclear & Cytoplasmic Staining fully solved 2024
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Histotechnology--Nuclear & Cytoplasmic Staining 
c. nuclei 
 
*hemalum stains (hematoxylin mordanted with either ammonium aluminum sulfate or potassium aluminum sulfate) are used almost exclusively to stain nuclei 
Harris hematoxylin is used on tissue sections to stain: 
 
a. fat 
b. glycogen 
c. nuclei 
d. cytoplasm 
 
 
b. oxidation 
 
*can be done naturally (atmospheric oxygen), or with oxidizing agents 
Ripening of hematoxylin is a process of: 
 
a. hydrolysis 
b. oxidation 
c. mordanting 
d...
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HISTOTECHNOLOGY with complete solution 2024
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HISTOTECHNOLOGYArt and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence or absence of a disease. 
Histotechnology 
 
 
Used to view slides not seen by the naked eye 
microscope 
 
 
Used by pathologist to view slides to identify a disease or process. 
microscope 
 
 
Used by histotechnologist to view slides FOR QUALITY CONTROL 
microscope 
 
 
Basic instrument used in microtomy (the process 
by which processed tissue, most common...
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Histotechnology question n answers graded A+
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Select the procedure suitable for demonstrating secretory granules in a carcinoid tumor: 
 
A. Wilder reticulum 
B. PAS-aniline blue 
C. Snook 
D. Sevier-Munger 
D. Sevier-Munger 
 
The Sevier-Munger technique is an excellent argyrophil procedure for demonstrating carcinoid tumors. It is similar to, but shorter than, the Grimelius. 
 
 
In Bodian's method for nerve fibers and neurofibrils, Protargol impregnates both neurofribrils and connective tissue. Subsequently, connective tissue is rendere...
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Histotechnology D2L Study Guide Exam Questions And Actual Answers.
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What thickness are paraffin sections routinely cut? - Answer 4-6 um 
 
Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? - Answer water bath that is too hot may distort tissue morphology 
 
Warm enough to relieve compression 
 
Cool enough so the wax does not melt 
 
What thickness are paraffin sections cut for the Luxol Fast Blue procedure? - Answer 10 - 15 um 
 
Why are paraffin blocks put on ice prior to cu...
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