Pcr product Samenvattingen, Aantekeningen en Examens

Types of prevention - answer-primary- car restraints, bicycle helmets, immunizations secondary- prevent for those with RF-pap, mammo tertiary-mgmt of established disease- meds, lifestyle Primary - answer-Preventing the health problem, most cost effective form of healthcare **IMMUNIZATIONS, ensuring adequate illumination at home (preventing falls) Secondary - answer-Detecting disease in early asymptomatic stages, screenings -Early cause finding of asymptomatic disease via the use of a sc...

RNAS C Practice Test Review 437 Questions with Verified Answers A capacitor and resisitor in parallel - CORRECT ANSWER will have same voltage across since in parallel. if in series, voltage drops would occur they can be treated together If the absolute pressure of a gas is increased from 3 atm to 4 atm at constant volume, then the absolute temperature of the gas will increase by: A. 25%. B. 33%. C. 67%. D. 75% - CORRECT ANSWER B. Assuming the validity of the Ideal-Gas law, PV = n...

E. coli RNA Polymerase II - Answer DNA template, product: mRNA RNA Polymerase I - Answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - Answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - Answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA Mammalian DNA polymerase α - Answer DNA template, product: primers HCV RNA Polymerase - A...

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Methylation: a primitive immune system for bacteria - hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) Methylation: a primitive immune system for bacteria - mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated - can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) pCR 2.1 - TOP...

WGU Biochemistry OA (Latest 2022/2023) Verified Answers Bohr Effect chart (low pH) C- High CO2 H- High H+ A- Acidic R-Release of O2(rt. Shift) T- tense state (in tissues) Primary structure Sequence of amino acids that make up a protein Secondary Structure Sequence of amino acids fold. Tertiary Structure -more folding in a 3D shape. -R groups (side chains) Quaternary Structure Protein has more than 1 polypeptide chain. Chemical reaction -absorb and release en...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

This refers to a means by which large quantities of DNA can be amplified from a small amount of DNA template: A. PCR B. DNA mass production C. DNA synthesis D. DNA birth correct answersPCR With regards to PCR, which of the following is NOT accurate? A. Denature strands at 94 degrees B. Base pairing of primers at 60 degrees C. Elongation of primers using Taq polymerase at 72 degrees D. Filtration of DNA at 40 degrees correct answersFiltration of DNA at 40 degrees Which of the follow...

American Heart Association protocols are based on __________. Select one: A. theories ready for field trial B. trends in the EMS community C. opinions from a core group of cardiologists D. evidence-based research - ANSWER D. evidence-based research As an EMT, you may be authorized to administer aspirin to a patient with chest pain based on: Select one: A. medical director approval. B. the patient's condition. C. an order from a paramedic. D. the transport time to the hospital. - AN...

Which level of protein structure is disrupted through the hydrolysis of peptide bonds? Quaternary Tertiary Primary Secondary - ANSWER Primary The primary structure of a protein is the sequence of amino acids held together by peptide bonds. Peptide bonds are formed by dehydration reactions and disrupted by hydrolysis. A mutation in the beta-hemoglobin gene, which results in the replacement of the amino acid glutamate in position 6 with the amino acid valine, leads to the develop...

BTEC Prep Exam 2 Questions & Answers 2024/2025 In drug regulation, indication refers to: - ANSWERSthe condition that could be treated by an approved drug What is true of biologics - ANSWERS"Biologics include growth factors, antibodies, and vaccines" Biologics is any natural or recombinant protein drug less than 40 amino acids long Biologics are generally stable even when subjected to high temperatures "Biologics are never made up of more than 10,000 atoms" FDA defines a drug as...