Ecorv Samenvattingen, Aantekeningen en Examens

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...

Een zeer uitgebreide samenvatting van het vak biochemie, gedoceerd door Prof Steyaert aan de 2e Ba chemie en Bio ingenieuren van de VUB.

BISC 440 Lab 5: Molecular Cloning questions and answers 100% accurate The RAD52 gene can be isolated from the yeast cells. What should we do first so we can isolate the gene we need? a. separate the DNA using gel electrophoresis b. perform DNA sequencing c. use a restriction enzyme d. perform DNA extraction d In order to isolate RAD52, what should we do after extracting all DNA from the yeast cells? a. sequence the DNA b. proceed to the ligation step c. transform the yeast cells ...

Lab Skills: convert .2 ml to microliters 200 ul Lab Skills: This type of molecule cannot form hydrogen bonds with water molecules and it repels water molecules. characteristics of hydrophobic molecules Lab Skills: Your total magnification on a light microscope if you are using the 100X objective 1000X Lab Skills: 0.0001 expressed in scientific notation 1.0 * 10^-4 Lab Skills: Formula used to make single step dilutions C1V1 = C2V2 Bacteria: cocci, bacilli...

Dit is een samenvatting van het hoorcollege over recombinant DNA technologie van MGOT. Onderwerpen die aan bod komen, zijn: kloneren, lambda, fagen, EcoRV, restrictie-modificatie systeem, endonuclease, restrictie enzym, type I, type II, ligatie, fosfatase, kloneringsplasmide, lacZ screening, vectoren, cDNA, genomische kloon, cDNA library, TOPO, gateway cloning, golden gate cloning etc.