Real time pcr Samenvattingen, Aantekeningen en Examens

MBB331 MIDTERM 1 - Spring 2017 ANSWER KEY NAME (PRINT in block letters):____________________________________________ SFU ID: ________________________________________________________________ TA/TUTORIAL DAY:TIME _______________________________________________ Answer all questions in the spaces provided. Keep your answers concise and legible. If your writing is not legible, you will not be given any marks for your answers. Exam total = 45 marks Section 1. Multiple choice and True/False ques...

Molecular Diagnostics questions with complete answers Define Molecular Diagnostics Use of DNA, RNA and protein aka amino acid, to test for specific disease What is one of the way molecular diagnostic are use in the lab and gave an example? To speed up the identification of 1) slow growing microrganism 2) Fastidious microrganisms 3) Microrganism that wont grow on conventional media> 01:03 01:30 What are the Molecular diagnostic Principle and describle each one? 1...

Uitgebreide en duidelijke samenvatting van moleculaire biologie en genomics.

Molecular Diagnostics Final (Exam + Quiz MC) /verified/ d (primers) *Ans* *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) *Ans* *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) *Ans* *A DNA se...

MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 What is the Beer-Lambert Law? the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: 260nm Proteins are absorbed at: 280nm What is the melting temperature equation? Tm = (4 x # of GC pairs) + (2 x # of AT p...

ASCP MB: Extra-Practice Questions with correct answers 2024 The Klenow fragment of E. coli DNA polymerase I is used: - answer-to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - answer-gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the prot...

MB ASCP Study Questions (1) Questions and Answered 100% correct Which of the following terms refers to the proportion of individuals affected by an autosomal dominant condition that displays manifestations? A. codominance B. penetrance C. expressivity D. influence E. passivity Answer Key: B penetrance Feedback: Certain autosomal dominant conditions are variably expressed due to differences in penetrance and expressivity. Penetrance refers to how people with the genetic abnormality mani...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

A; Animals serve as a reservoir of infection" is the correct answer because Shigella only infects humans while Salmonella is found in many animals. Both have diarrhea as the major symptom. Endotoxin is produced by all gram negative bacteria and exotoxin is only produced by Shigella. A major difference between Salmonella enterica and Shigella infections is that only in salmonellosis can: A. animals serve as a reservoir of infection B. diarrhea become the major symptom C. endotoxin play a ...

(MB) ASCP Exam What kind of bond do histones have with DNA? - Answer Ionic What amino acids compose histones? - Answer Lysine and Arginine Which lymphoma has t-cell rearrangements? - Answer Sezary Syndrome Acute Promyelocytic leukemia translocation? - Answer t(15,17) Leading strand polymerase: - Answer Delta DNA repair polymerase(s): - Answer Beta or Epsilon Mitochondrial polymerase: - Answer Gamma Molecular beacon design: - Answer (R-sequence-Q) Which method uses v...