Bright field microscopy Samenvattingen, Aantekeningen en Examens

What are the 2 most common measurements used in microscopy - micrometer and nanometer describe simple microscopes and compound microscopes - simple microscopes contain a single lens; compound microscopes contain a group of diverse lenses What is refracted light - "light whose rays have been bent out of their original course by passing through a transparent membrane" list 3 basic lens shapes - flat, convex, and concave what is the "resolving power" of a microscope lens - ability to dis...

Hooke -coined term cells -made first microscope Spontaneous generation Living organisms appear from non-living matter Needham -for spontaneous generation -boiled uncovered broth and it became cloudy Spallanzani -against spontaneous generation -did one broth uncovered and one covered and boiled each Pasteur -against spontaneous generation -swan neck flask -pasteurization -silk worm Koch (not postulates) -link between microbe and disease -bacillus an...

Resolution - small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy - normal light type Phase-contrast - altered phase interference of unobstructed light; looks like cells are surrounded by light DIC - 3D looking cells Confocal - 3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs. spinning disk

Bio 315 Exam 1 Questions and Answers 100% Pass Cytology - Correct Answer ️️ -focuses mainly on cellular structure and emphasizes optical techniques Biochemistry - Correct Answer ️️ -focuses on cellular structure and function Genetics - Correct Answer ️️ -focuses on information flow and heredity and includes sequencing of the entire genome (all of the DNA) in numerous organisms Robert Hooke - Correct Answer ️️ -Discovered cells 30x magnification -termed cells Cellula -drew...

What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer) - 60 x 10 = 600x magnification True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy - False. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast Which of the following could be seen clearly by the unaided eye? (multiple choice) - Protozoa with diameter of 150um and skin cell with diameter ...

Cytology - Answer-focuses mainly on cellular structure and emphasizes optical techniques Biochemistry - Answer-focuses on cellular structure and function Genetics - Answer-focuses on information flow and heredity and includes sequencing of the entire genome (all of the DNA) in numerous organisms Robert Hooke - Answer-Discovered cells 30x magnification -termed cells Cellula -drew cork as seen from a microscope -categorized as Cytology Van Leeuwenhoek - Answer-"Father of microbiology" -...

3 branches of microscopy - correct answers optical, electron, scanning probe 5 steps of sample preparation - correct answers fixation, processing, embedding, sectioning, and staining most common form of microscopy - correct answers bright-field light microscopy 3 types of optical microscopes - correct answers bright-field, phase contrast, fluorescent bright-field microscope - correct answers light microscopy, halogen/LED bulb is light source, compound upright objective turret=most co...

Function of centrifuge - ANSWERSeparates substances based on density Daily maintenance of centrifuge - ANSWERclean with 10% bleach periodic maintenance of centrifuges - ANSWERcheck speed using tachometer types of pipettes - ANSWERMeasuring, Serological, and Volumetric types of serological pipettes - ANSWERblow out and drain out types of thermometers - ANSWERliquid and glass and digital electronic temperature of heat block/water bath - ANSWER36-38 C temperature of incubators ...

Three Domains Correct Answer Archea, Bacteria, Eukarya Hierarchy Correct Answer Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species Zone of inhibition Correct Answer The clear region around the paper disc saturated with an antimicrobial agent on the agar surface. The clear region is an indication of the absence, or the effective inhibition, of microbial growth by the antimicrobial agent. Koch's Postulates Correct Answer 1. The suspected causative agent must be found in eve...

What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer) - 60 x 10 = 600x magnification True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy - False. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast Which of the following could be seen clearly by the unaided eye? (multiple choice) - Protozoa with diameter of 150um and skin cell with diameter ...