Southern blot Samenvattingen, Aantekeningen en Examens
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ASCP Exam Q&A
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Which is required to generate cDNA 
A. DNA polymerase 
B. RNase H 
C. Restriction Enzyme 
D. Helicase - Answer- B 
 
What is the function of Taq Polymerase - Answer- recognize dsDNA 
 
You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How much stock should you add(what is the dilution factor) - Answer- 2 
 
What gene causes hypersensitivity to Irinotecan? - Answer- UGT1A 
 
Which allele causes hypersensitivity to Carbamazepine? - Answer- HLA-b*1502 
 
What gives MLPA ...
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MB ASCP Test Molecular Techniques (20-30%) Questions and Answers All correct
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MB ASCP Test Molecular Techniques (20-30%) Questions and Answers All correct 
What are the 3 types of gel electrophoresis 
Agarose 
Polyacrylamide 
Pulse Field 
 
 
 
What is gel electrophoresis 
the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. 
 
 
 
which direction does the DNA flow in gel electrophoresis 
due to DNA being negatively charged, it will migrate towards the positive end 
 
 
 
What determines how far the DN...
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ASCP MB 2023/2024 ALREADY CORRECTLY ANSWERED -GRADED A+
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E. coli RNA Polymerase II - Answer DNA template, product: mRNA RNA Polymerase I - Answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - Answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - Answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA Mammalian DNA polymerase α - Answer DNA template, product: primers HCV RNA Polymerase - Answer RNA template, prod...
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ASCP MB 2023/2024 ALREADY CORRECTLY ANSWERED -GRADED A+
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ASCP MB 2023/2024 
 
 
E. coli RNA Polymerase II - Answer DNA template, product: mRNA 
 
RNA Polymerase I - Answer DNA template 
Location: Nucleous 
Products: 18s, 5.8s, 28s rRNA 
 
RNA Polymerase II - Answer DNA template 
Location: Nucleus 
Products: mRNA, snRNA 
 
RNA Polymerase III - Answer DNA template 
Location: Nucleus 
Product: tRNA, 5s rRNA, snRNA 
 
Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA 
 
Mammalian DNA polymerase α - Answer DNA template, product: primers 
...
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ADVANCED PLACEMENT (AP) BIOLOGY: Transcription 32 Questions Correctly Answered | 100% Rated
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ADVANCED PLACEMENT (AP) BIOLOGY: 
 
Transcription 
32 Questions Correctly Answered | 100% Rated 
Question 1 
Which of the following statements about post-transcriptional processing is true? 
Possible Answers: 
A methylguanine cap is added to the 3' end of the mature mRNA 
Exons are spliced out of hnRNA and are not part of the mature mRNA 
Post-transcriptional hnRNA processing prevents RNA from degrading as it leaves the nucleus 
A poly-A tail is added to the 5' end of the mature mRNA 
Cor...
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MB (ASCP) Practice Exam 2024 100% Correct
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MB (ASCP) Practice Exam 2024 100% Correct 
What does barcoding do in Next Generation Sequencing? - Answer ️️ -Adds unique tag to all 
nucleic acids within one sample so that various samples can be mixed 
What is the best way to store RNA for longer than a month? - Answer ️️ -In Ethanol, at -20°C 
or -80°C 
Testing for Aldehyde Dehydrogenase deficiency is important in patients with: - Answer ️️ - 
Alcohol dependence 
During DNA replication, the 3' -OH of the growing DNA chain attac...
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QBM Exam 3 Questions And Answers With Complete Solutions 2024
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QBM Exam 3 Questions And Answers With 
Complete Solutions 2024 
Chapter 13 - DNA Purification, Agarose Gel Electrophoresis, and DNA Visualization - answer 
The genetic information of all living organisms is contained within the sequence of ____ ____ 
= Examples include: purines and pyrimidines 
= For most organisms, the genetic information is contained in nucleic acids of the 
deoxyribonucleic acid (DNA), or ribonucleic acid (RNA) in certain viruses - answernucleic 
acids 
______ is a complete s...
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MB (ASCP) Exam Questions and Answers Graded A
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MB (ASCP) Exam Questions and Answers Graded A 
Predominant form of DNA - Answer ️️ -Right-handed B 
DNA and histone interaction is due to what type of bond? - Answer ️️ -Ionic 
What stains to the minor groove of DNA duplex? - Answer ️️ -SYBR Green 
What is the most abundant type of RNA? - Answer ️️ -Ribosomal RNA (rRNA) 
What does RNA act as in a retrovirus? - Answer ️️ -Genome and mRNA 
What increases half-life of mRNA? - Answer ️️ -5' Methyl Cap 
What kind of DNA is mo...
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ASCP Technologist in Molecular Biology board exam prep with complete solution 2023/2024
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ASCP Technologist in Molecular Biology board exam prep with complete solution 2023/2024 
 
Pyrimidine 
One carbon ring 
Cytosine, Thymine, Uracil 
Purine 
Two carbon rings 
Adenine, Guanine 
How are nucleotides joined together? 
Condensation to form phosphodiester bond 
What is the function of mRNA? 
Carries genetic info out of nucleus 
Transcript translated to protein 
What is the function of tRNA? 
Carries aa to ribosome 
Anticodon pairs with codon on mRNA strand 
What is the function of rRNA?...
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MB (ASCP) Exam Questions and Answers Graded A
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MB (ASCP) Exam Questions and Answers Graded A 
Predominant form of DNA - Answer ️️ -Right-handed B 
DNA and histone interaction is due to what type of bond? - Answer ️️ -Ionic 
What stains to the minor groove of DNA duplex? - Answer ️️ -SYBR Green 
What is the most abundant type of RNA? - Answer ️️ -Ribosomal RNA (rRNA) 
What does RNA act as in a retrovirus? - Answer ️️ -Genome and mRNA 
What increases half-life of mRNA? - Answer ️️ -5' Methyl Cap 
What kind of DNA is mo...
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