Real time pcr Samenvattingen, Aantekeningen en Examens

ASCP MB: Extra-Practice Questions & Answers 2024 The Klenow fragment of E. coli DNA polymerase I is used: - ANSWER-to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - ANSWER-gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucle...

Een samenvatting van het vak DNA Technologie & Ethiek wat in jaar 2 periode 4 wordt gegeven - opleiding BML. Het is een samenvatting over de lesstof, PowerPoints & aantekeningen van verschillende docenten/ professors. Inclusief uitwerkingen van het werkcollege!

What is the Beer-Lambert Law? - Answer the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? - Answer A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: - Answer 260nm Proteins are absorbed at: - Answer 280nm What is the melting temperature equation? - Answer Tm = (4 x # of GC pairs) + (2 x # of AT pairs) Dilutions equation - Answer V1C1 = V2C2 V1:...

The invention of the thermal cycler The use of reverse transcriptase from single-stranded RNA The use of fluorescently-tagged probes for real-time quantitation of PCR products - Answer- SIgnificant Advances in PCR include the structure of DNA was described, as well as its method of replication - Answer- What happened in the 1950s? the isolation of restriction enzymes - Answer- The advent of recombinant DNA technology began with what? it allowed us to manipulate DNA - Answer- What did...

The advantages of using real-time PCR to detect Chlyamidia and Gonorrhea is... - Answer - allow detection of organisms that are hard to culture - can be automated for high volume processing MALDI-TOF improves traditional culture methods by... - Answer -reduces need for biochem tests -pathogen-same method for all bacteria and fungi -cheaper cost per sample Describe the precautions that the State lab takes to prevent contamination (3) - Answer -designated clean (pre) and dirty (pos...

ASCP Exam Questions and Answers Actual Test 100% Correct Which is required to generate cDNA A. DNA polymerase B. RNase H C. Restriction Enzyme D. Helicase B What is the function of Taq Polymerase recognize dsDNA You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How much stock should you add(what is the dilution factor) 2 What gene causes hypersensitivity to Irinotecan? UGT1A Which allele causes hypersensitivity to Carbamazepine? HLA-b*1502 What gives ...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: - Answer ️️ -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer ️️ -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to...

ASCP PRACTICE EXAM 2024 WITH 200 ACTUAL EXAM QUESTIONS AND DETAILED 100% CORRECT ANSWERS | SCORED A+ PCR that is not a limitation of real-time PCR? Select one: a. Requirement of a large initial sample b. Inability to quantitate DNA product due to endpoint analysis only c. Ability to detect PCR product during the plateau stage - ANSWER- B. Inability to quantitate DNA product due to endpoint analysis only A patient has the following test results:ANA = positive 1:320RA = positiveComplemen...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: - Answer ️️ -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer ️️ -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to...

dCE - Answer-ry DCE - Answer-RZ Hardy Weinberg equation - Answer-Used to calculate allele and genotype frequencies in a population when the frequency of one genetic trait is known HLA antigens - Answer-Certain characteristics of blood group and ____________ antigens make them useful tools for parentage analysis Direct exclusion, indirect excursion - Answer-Assuming maternity is correct, paternity can be excluded in one of two ways Genetic markers present in the child but is absent from ...