Identification of Ascarophis sp. in
Scorpaena porcus by morphological
analysis
Bachelor of Agro- and Biotechnology
specialization Biotechnology
Bachelor's thesis
Student: Decaluwe Matisse
Academic year 2021-2022
Supervising lecturer: Jan Van Doorsselaere
Internship mentors: Ana Pérez-del-Olmo, Francisco E. Montero,
Marta Valmaseda Angulo
Instituto Cavanilles De Biodiversidad y Biología Evolutiva
Catedrático José Beltrán n°2
46980 Paterna, Valencia, Spain
http://zoomar.blogs.uv.es
,Identification of Ascarophis sp. in Scorpaena porcus by morphological analysis Science Park University of Valencia 1
Identification of Ascarophis sp. in Scorpaena
porcus by morphological analysis
Decaluwe Matisse academic year 2021-2022
,Identification of Ascarophis sp. in Scorpaena porcus by morphological analysis Science Park University of Valencia 2
Abstract
Purpose The objective of this bachelor thesis is the morphological identification of Ascarophis
sp. in Scorpaena porcus from a local museum sample. The identification of Ascarophis sp.
occurred by the use of different microscopical techniques.
Methods Throughout three months, 24 specimens of S. porcus were used for dissection and
analysis. The S. porcus specimens were caught in two locations, Punta Maricones and
Chafarinas Islands (Northern Africa). The nematode specimens were identified with the use of
both optical microscopy techniques and a scanning electron microscope (SEM).
Results The specimens of Ascarophis sp. were obtained of S. porcus from the stomach wall,
stomach lumen, or intestine. The total length of the Ascarophis sp. males was measured:
9.73mm long and 0.08mm width. The left spicule was measured, with a length of 319.7µm and
the right spicule at 105.5µm.
Conclusion The Ascarophis sp. founded in S. porcus is A. filiformis. By comparing the
locations and hosts on different databases such as WORMS, and EOL, a second conclusion
was made. A. filiformis has a new host, S. porcus , and a new location, the Mediterranean Sea.
Keywords: Scorpaena porcus | Mediterranean Sea | Parasitic nematode |
Ascarophis filiformis | Morphological identification
Decaluwe Matisse academic year 2021-2022
, Identification of Ascarophis sp. in Scorpaena porcus by morphological analysis Science Park University of Valencia 3
Preface
During my internship and working on my bachelor thesis I acquired knowledge about fish
parasites and learnt how fish parasites are morphologically different from each other and how
to recognize these parasites.
I would like to say thanks to some people. First of all, I would like to thank the University of
Valencia Science Park for allowing me to do my internship and work on my bachelor thesis.
Secondly, I would like to say thanks to my mentors Professor Francisco E. Montero, Professor
Ana Pérez del-Olmo, and Ph.D. Marta Valmaseda Angulo for helping and reading my thesis,
and for the guidance during practical experiments in the lab. Thank you to my mentor sir Jan
Van Doorsselaere (Vives Roeselare, Belgium) for reading through my bachelor thesis and the
guidance during my internship.
I would like to say thanks to all of the co-workers at the lab, for helping me, and answering my
questions when my mentors were not around.
Finally, I want to say thank you to my family, specifically my parents. They allowed me to go
to Erasmus, they gave me the chance to study Biotechnology and I appreciate everything they
gave done for me over the three years.
Decaluwe Matisse
23 February 2022
Decaluwe Matisse academic year 2021-2022
Scorpaena porcus by morphological
analysis
Bachelor of Agro- and Biotechnology
specialization Biotechnology
Bachelor's thesis
Student: Decaluwe Matisse
Academic year 2021-2022
Supervising lecturer: Jan Van Doorsselaere
Internship mentors: Ana Pérez-del-Olmo, Francisco E. Montero,
Marta Valmaseda Angulo
Instituto Cavanilles De Biodiversidad y Biología Evolutiva
Catedrático José Beltrán n°2
46980 Paterna, Valencia, Spain
http://zoomar.blogs.uv.es
,Identification of Ascarophis sp. in Scorpaena porcus by morphological analysis Science Park University of Valencia 1
Identification of Ascarophis sp. in Scorpaena
porcus by morphological analysis
Decaluwe Matisse academic year 2021-2022
,Identification of Ascarophis sp. in Scorpaena porcus by morphological analysis Science Park University of Valencia 2
Abstract
Purpose The objective of this bachelor thesis is the morphological identification of Ascarophis
sp. in Scorpaena porcus from a local museum sample. The identification of Ascarophis sp.
occurred by the use of different microscopical techniques.
Methods Throughout three months, 24 specimens of S. porcus were used for dissection and
analysis. The S. porcus specimens were caught in two locations, Punta Maricones and
Chafarinas Islands (Northern Africa). The nematode specimens were identified with the use of
both optical microscopy techniques and a scanning electron microscope (SEM).
Results The specimens of Ascarophis sp. were obtained of S. porcus from the stomach wall,
stomach lumen, or intestine. The total length of the Ascarophis sp. males was measured:
9.73mm long and 0.08mm width. The left spicule was measured, with a length of 319.7µm and
the right spicule at 105.5µm.
Conclusion The Ascarophis sp. founded in S. porcus is A. filiformis. By comparing the
locations and hosts on different databases such as WORMS, and EOL, a second conclusion
was made. A. filiformis has a new host, S. porcus , and a new location, the Mediterranean Sea.
Keywords: Scorpaena porcus | Mediterranean Sea | Parasitic nematode |
Ascarophis filiformis | Morphological identification
Decaluwe Matisse academic year 2021-2022
, Identification of Ascarophis sp. in Scorpaena porcus by morphological analysis Science Park University of Valencia 3
Preface
During my internship and working on my bachelor thesis I acquired knowledge about fish
parasites and learnt how fish parasites are morphologically different from each other and how
to recognize these parasites.
I would like to say thanks to some people. First of all, I would like to thank the University of
Valencia Science Park for allowing me to do my internship and work on my bachelor thesis.
Secondly, I would like to say thanks to my mentors Professor Francisco E. Montero, Professor
Ana Pérez del-Olmo, and Ph.D. Marta Valmaseda Angulo for helping and reading my thesis,
and for the guidance during practical experiments in the lab. Thank you to my mentor sir Jan
Van Doorsselaere (Vives Roeselare, Belgium) for reading through my bachelor thesis and the
guidance during my internship.
I would like to say thanks to all of the co-workers at the lab, for helping me, and answering my
questions when my mentors were not around.
Finally, I want to say thank you to my family, specifically my parents. They allowed me to go
to Erasmus, they gave me the chance to study Biotechnology and I appreciate everything they
gave done for me over the three years.
Decaluwe Matisse
23 February 2022
Decaluwe Matisse academic year 2021-2022
