Bios 390 week 1 lab 1 Study guides, Revision notes & Summaries

BIOS 390 Week 1 LAB 1

BIOS 390 Week 1 LAB 1

BIOS 390 ERIC KYLE WEEK 3 & 4 : LAB DNA cloning is the process of making multiple, identical copies of a certain piece of DNA. A gene or other DNA fragment of interest is first inserted into a circular piece of DNA called a plasmid. The insertion is done using enzymes that are able to “cut and paste” DNA, and then a molecule of recombinant DNA is produced. Next, the recombinant plasmid is introduced into bacteria. Bacteria carrying the plasmid are selected and grown up. As they reproduce, ...

BIOS 390 ERIC KYLE WEEK 3 & 4 : LAB DNA cloning is the process of making multiple, identical copies of a certain piece of DNA. A gene or other DNA fragment of interest is first inserted into a circular piece of DNA called a plasmid. The insertion is done using enzymes that are able to “cut and paste” DNA, and then a molecule of recombinant DNA is produced. Next, the recombinant plasmid is introduced into bacteria. Bacteria carrying the plasmid are selected and grown up. As they reproduce, ...

BIOS 390 WEEK 5: LAB: transcription in prokaryotes Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make an RNA molecule. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble. Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA product is complementary to the ...

BIOS 390 WEEK 5: LAB: transcription in prokaryotes Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make an RNA molecule. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble. Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA product is complementary to the ...

Polymerase chain … (PCR) is a laboratory technique to increase, or make many copies of, a specific target region of DNA in vitro. The goal of PCR is to make enough of the target DNA region that it can … analyzed or used in some other way. For example, DNA amplified by PCR may … sent for sequencing, visualized by gel electrophoresis, or cloned into a plasmid for further experiments…………………..document continueBIOS 390 Week 1 LAB 1

Question Set 1 (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plan ts and then allowed the offspring to self-pollinate to produce an F2 The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what relationship to the allele for yellow seeds? (TCO 1) In 1953, James Watson and Francis Crick proposed. Question Set 2 (TCO 1) In...

BIOS 390 Week 1 LAB 1: Polymerase Chain Reaction, BIOS 390 Week 2 LAB 2: Gel Electrophoresis

BIOS 390 Week 1 Quiz Study Guide Question Set 1 • (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: • (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plants and then allowed the offspring to self-pollinate to produce an F2 The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what relationship to the allele for yellow seeds? • (TCO 1) In 1953, James Watson and Franc...