Dna plasmid Study guides, Class notes & Summaries

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

MCB 3020C Module 2 Exam Review Questions and Answers 2024. *Anything denoted in red are extra important takeaways 1. What is the function of the Z ring in binary fission? A. It forms a contractile ring at the septum => Made up of FtsZ (tubulin homologues) molecules important for bacterial septation B. It controls the replication of DNA => Replisome contains DNA replication machinery C. It separates the newly synthesized DNA molecules => ParA polymerizes/depolymerizes to attach t...

OpenStax Microbiology Test Bank Chapter 12: Modern Applications of Microbial Genetics * = Correct answer Multiple Choice 1. Which of the following is the definition of biotechnology? A. The alteration of DNA in a cell B. The use of living systems to benefit humankind* C. Using computers to synthesize biomolecules D. Using DNA for solving mathematical problems 2. Which of the following is the definition of genetic engineering? A. The alteration of DNA in a cell* B. The use of livin...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

IB Biology 1.2 Latest Update Already Passed Resolution Resolution is defined as the shortest distance between two points that can be distinquished Explain why electron microscopes have a better resolution than light microscopes because the wavelength of the electrons is thousands of times smaller than the wavelength of light. Ultrastructure all the structures of a biological specimen that are at least 0.1 nm in their smallest dimension Something that electron microscopes can se...

BIO 205 Exam Questions With 100% Correct Answers Nucleoid is a region/structure found only in ____ cells. a) prokaryotic b) eukaryotic c) bacterial d) Both A and C - answerProkaryotic/bacterial A plasmid is double-stranded DNA. True or false? - answerTrue A plasmid-gene or a chromosomal-gene (piece of DNA with a specific product) will always produce a________. a) carbohydrate b) ATP c) Protein d) Lipid - answerProtein DNA polymerase is a(n) a) lipid b) polysaccharide c) enzyme ...

Name the two types of Eukaryotic cells - ANSWER Plant cells, animal cells What type of cells are Prokaryotic cells? - ANSWER Bacteria cells Where are chromosomes found in an animal cell - ANSWER Nucleus Describe the DNA in a bacteria cell - ANSWER A single DNA loop or a DNA ring (plasmid) What is 'centi' in standard form? - ANSWER 10 to the power of -2

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample...

structure of DNA - Answer-composed of nucleotide sub units - has sugar - phosphate backbone (deoxyribose and phosphate) DNA - Answer-deoxyribosenucleic acid nitrogen - containing bases - Answer-adenine, guanine, thymine, cytosine adenine, guanine, thymine, cytosine - Answer-held together by hydrogen bonds adenine = thymine guanine = cytosine - Answer-always pair together (DNA) DNA replication - Answer-- 1 parent has 2 strands of separates -each strand used to make new strand - 2 daughte...

m   1. Which of the following is the mRNA start codon in most cases? A. UAA B. AGU C. AUG D. UGA 2. Which of the types of RNA is the smallest? A. mRNA B. tRNA C. rRNA D. 3. Which of the following is not considered a pyrimidine? A. C B. T C. U D. G 4. Which of the following is paired correctly? A. A-G B. C-G C. A-U D. G-T 5. Which of the following characterizes a Western blot? A. Antibody/protein hybridization B. DNA/RNA combination C. RNA ...