Molecular diagnostics Study guides, Class notes & Summaries

Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics 7th Edition Test Bank

Which double-stranded DNA molecule has the highest melting temperature? A. An oligonucleotide with a repeating sequence of A-A-A at the 5 ́ end B. A molecule of 5,000 base pairs with a high number of A-T base pairs C. An oligonucleotide with a large number of repeating C-G-C codons D. A DNA polymer of 100,000 base pairs - Answer C. The melting temperature of DNA refers to the temperature required to separate the molecule into single strands. The Tm is the temperature required to convert h...

What action should be taken if an unlabeled collection tube with a requisition for a factor V Leiden test is received in the laboratory. - Answer Notify the supervisor and reject the specimen. What action should be taken if after PCR, the amplification control has failed to yield a product? - Answer Check the original DNA or RNA preparation. If it is adequate, repeat the amplification. If not, reisolate the nucleic acid. What action should be taken if an isolated DNA sample is to be st...

The analysis of external specimens to assess technologist competency and assay performance (CAP survey) is which kind of testing? - Answer Proficiency A woman calls and asks that the results of her husband's HIV viral load assay be faxed to her. She gives the technologist her husband's name and medical record number along with the date the specimen was collected and her fax number. She states that she is calling for her husband and has his approval. Which of the following is the correct c...

Test Bank For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics 7th Edition by Carl A. Burtis

TEST BANK For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai, Verified Chapters 1 - 49, Complete Newest Version TEST BANK For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai, Verified Chapters 1 - 49, Complete Newest Version TEST BANK For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai, Verified Chapters 1 - 49, Complete Newest Version

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons? - Answer to find informative alleles for post-transplantations studies Two elderly women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analysis would give the best information toward proving their maternal lineage? - Answer Mitochondrial DNA A PCR assay usin...

in one nucleotide of DNA what are the 3 major moieties (components) that make it up? - Answer - 5 carbon sugar - phosphate group - nitrogen bases what is the overall charge of DNA and on which moiety is it found - Answer DNA is overall negative, it is found on the oxygen of phosphate backbone name a method that uses the fact that DNA is charged to get that method to work? - Answer Agarose gel electroporesis what types of bonds are formed during hybridization when a strand of DNA...

A micoarray is testing for: - Answer a lot of genetic characteristics from one sample Southern Hybridization - Answer extract DNA probe w/ DNA Northern Hybridization - Answer extract RNA probe w/ DNA Western Hybridization - Answer extract proteins probe with antibodies What is the first method that became standard in DNA sequencing? - Answer Sanger method What type of PCR does Sanger Method use? - Answer cycle sequencing PCR Cycle Sequencing PCR requirements -...

TEST BANK For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai, Verified Chapters 1 - 49, Complete Newest Version Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai TEST BANK For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition