Molecular Diagnostics questions with correct answers

The analysis of external specimens to assess technologist competency and assay performance (CAP survey) is which kind of testing? - Answer Proficiency A woman calls and asks that the results of her husband's HIV viral load assay be faxed to her. She gives the technologist her husband's name and medical record number along with the date the specimen was collected and her fax number. She states that she is calling for her husband and has his approval. Which of the following is the correct course of action for the technologist? - Answer Tell the woman that her husband can get his test results from his physician. Which of the following is an example of an ASR: - FastTrack RNA extraction kit (Invitrogen) - Edvotek PCR bead (pellet) - Roche LightCycler for real time PCR - PCR primer for PV92 locus - Answer PCR primer for PV92 locus An example of a unique clinical molecular QC procedure is - Answer always running a no-template PCR control In the differential PCR for the assessment of Her2/neu amplification in a patient with breast cancer the beta actin gene is co-amplified alongside the Her2/neu. What is the purpose of incorporating beta actin into this assay? - Answer It serves as an internal amplification control run to exclude the presence of PCR inhibitors in the sample AND it serves as a quantitative amplification standard CLIA regulations that usually apply to molecular diagnostics lab fall into which category? - Answer High complexity testing