BOC Histotechnology- Processing correctly answered graded A+ 2023/2024 latest update

BOC Histotechnology- Processing During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be achieved by fixation in: - correct answers ZENKER SOLUTION. Because Zenker contains acetic acid it can decalcify needle biopsies of bone marrow. A major disadvantage of aliphatic clearing agents is that they: - correct answers ARE INCOMPATIBLE WITH SOME MOUNTING MEDIA. Aliphatic xylene substitutes are incompatible with mounting media, because mounting media contain toluene or xylene. Which of the following is MOST likely to cause sensitization with prolonged use? - correct answers LIMONENE. Limonene causes sensitization and allergic reactions in some individuals. To speed up the laboratory's processing of all surgical tissues, the temperature of all fixation, dehydration, and clearing steps has been set to 45 degrees Celsius. This will most likely result in: - correct answers MICROCHATTER. Excess heat during processing, especially in dehydration and clearing, can lead to removal of bound water and results in micro chatter during microtomy. Limonene functions as a: - correct answers CLEARING AGENT ONLY. Limonene is used as a xylene substitute, making it a clearing agent. One advantage of aliphatic hydrocarbons is that they: - correct answers ARE LOW IN TOXICITY AND SENSITIZATION. Aliphatic hydrocarbons are less toxic and lead to less sensitization than aromatic hydrocarbons used as clearing agents. A disadvantage of using heat at all stations of the tissue processor is that it will: - correct answers HARDEN SOME TISSUES. When heat is used in all stations of the processor (especially dehydration and clearing), the tissues become hard. Dehydration refers to the removal of: - correct answers WATER. Which decalcification method may cause heat damage to the specimen? - correct answers ELECTROLYTIC. Electrolytic methods of decalcification may cause damage to the specimen because of the heat generated by the method. Which o the following clearing agents is NOT flammable? - correct answers CHLOROFORM. Chloroform is not flammable or combustible, but when heated, it may form a toxic gas. Which of the following groups of reagents may be used for dehydration? - correct answers DIOXENE, METHANOL, AND ETHANOL.