Gfp Study guides, Class notes & Summaries

BIOD 171 Portage Learning Final EXAM Questions and Answers 100%Correct/Exam 1-7 2023/2024 UpdatePortage Learning BIOD 171 Final EXAM Questions and Answers 100%Correct/Exam 1-7 2023/2024 Update True or False: A virus is considered a microorganism. False. Viruses are not living and as such are not considered microorganisms. Viruses can, however, be classified as microbes, a more general term that includes microorganisms and viruses. 1. What is the smallest biological unit of life? A cell. ...

What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer) - Answer 60 x 10 = 600x magnification True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy - Answer False. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast Which of the following could be seen clearly by the unaided eye? (multiple choice) - Answer Protozoa with diameter of 150um ...

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...

LAB 9 - Green Fluorescent Protein (GFP) PurificationWhat is a protein? Proteins are long chains of amino acids, some of which are hydrophobic Explain the relationship between genes and proteins: Genes contain the genetic code which determine the amino acid composition of a protein. There is a unique gene for each protein within all of the cells of the body Using your own words, describe cloning: The duplication and propagation of a cell or organism. Describe how the bacte...

PURIFICATION OF GFP FUSION PROTEINS WITH IMAC IN E. Coli CELLS ABSTRACT This report was performed to demonstrate techniques of purifying Green Fluorescent Protein (GFP). The main technique used for demonstration is by Immobilized Metal Affinity Chromatogrpahy (IMAC). The open reading frame for enhanced green fluorescent protein was subcloned into the pQE30 expression. The subsequent production of protein tagged N-terminally with hexahistidine, facilitated the purification by IMAC

Resolution Correct Answer-small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy Correct Answer-normal light type Phase-contrast Correct Answer-altered phase interference of unobstructed light; looks like cells are surrounded by light DIC Correct Answer-3D looking cells Confocal Correct Answer-3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs....

Contracting Officer (CO) - ANSWER A Federal employee delegated authority to award, administer, and terminate contracts, purchase orders, delivery orders, task orders and modifications; obligate Government funds; and make determinations and findings, all subject to the limitations of his or her Certificate of Appointment. Contracting Officer's Representative (COR) - ANSWER A Federal employee appointed in writing, delegated limited responsibilities by a CO to perform specified contract managem...

Counseling and Feedback TLOs (Just a guide for what to takeaway from this section) 1. Review the four stages of counseling in the correct sequence. 2. Analyze at least ten forms of resistance. 3. Explain how counseling relates to leader development in at least three ways First Stage of Counseling Identify the Need for Counseling (require evaluation or command-directed counseling, counsel whenever focused, two-way communication aimed at the subordinate's development is needed) ...

Principles of immunofluorescence staining we want to identify 2 dif proteins, how do we do it? *Make sure that ........ (2 essential parts to note) - First we must generate primary antibodies that recognize/bind our target protein from 2 DIFFERENT species animals. then, use secondary antibody (tagged with color) from a 3rd animal that recognizes/binds the primary but is a dif color than the other secondary antibody *primary antibodies must be from different species *secondary anti...

Contracting Officer Representative (COR) 101 Questions and Answers 100% Pass Contracting Officer (CO) A Federal employee delegated authority to award, administer, and terminate contracts, purchase orders, delivery orders, task orders and modifications; obligate Government funds; and make determinations and findings, all subject to the limitations of his or her Certificate of Appointment. Contracting Officer's Representative (COR) A Federal employee appointed in writing, delegated limite...