Cloning vectors Study guides, Class notes & Summaries

RNAS-C Exam 4 Practice Test 75 Questions with Verified Answers A couple expecting their second child is worried about Rh incompatibility. Their first child was Rh+. They have their blood typed. Which result would be cause for alarm? A) both parents Rh- B) both parents Rh+ C) man Rh- and woman Rh+ D) man Rh+ and woman Rh- - CORRECT ANSWER D) man Rh+ and woman Rh- Research indicates that allergies may actually be due to a mutation in the gene that encodes a skin protein called __________...

Biotechnology |Cloning Vectors Questions and Answers

immunological assay method correct answers What method do we use when we want to find a protein we are interested in but we don't know the exact genetic sequence? antibody binding correct answers What mechanism does the immunological assay method use to highlight the desired protein? primary antibody correct answers In the immunological assay method, the ____ binds to the target protein. secondary antibody correct answers In the immunological assay method, the ____ reacts with the ...

GCD Exam 3 Questions with 100% Pass Which of the following may be used as a vector in a gene-cloning experiment? a) mRNA b) Plasmid c) Virus d) c & d - Answer-d The restriction enzymes used in gene-cloning experiments ___________, which generates sticky ends that can _________. a. cut the DNA, enter bacterial cells b. cut the DNA, hydrogen bond with complementary sticky ends c. methylate DNA, enter bacterial cells d. methylate DNA, hydrogen bond with complementary sticky ends - Answe...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

All of the following are characteristics that make plasmids good cloning vectors, except A. they are small B. they are well characterized C. they can be transferred into appropriate host cells D. they can carry genetic markers, such as antibiotic resistance genes E. they can accept a relatively large amount of foreign DNA Ans: E. they can accept a relatively large amount of foreign DNA Types of Plasmids: -Fertility Factors -Resistance Factors -Bacteriocin Factors -Virulence Plasmids ...

EPPN 154 Exam 3 Questions & Answers 2024/2025 Expressing foreign genes in bacteria - ANSWERSTranscription and translation to produce coded proteins Obstacles in expressing foreign genes in bacteria - ANSWERS1. Genes placed under control of a bacterial promoter 2. Introns must be removed 3. Codons might need editing 4. Many eukaryotic proteins require host modification after translation Expression Vectors allow for control of what? - ANSWERSexpression of the cloned genes How mus...

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

MCB Exam 3 Questions & Answers 2023/2024 What is meant by recombinant DNA? - ANSWER-DNA w/fragments from 2+ sources Name the tools used to make a recombinant DNA. (mechanisms of gene cloning) - ANSWER-1) Isolate plasmid DNA from bacterial cell 2) Isolate DNA of interest 3) Insert gene of interest into plasmid DNA 4) Insert now recombinant DNA into bacterium 5) Culture bacteria to make copies Restriction enzymes - ANSWER-endonuclease that cuts DNA at specific sites Cloning vecto...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...