Tsi agar slant Study guides, Class notes & Summaries
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Microbiology Practical Review- Carbohydrate Catabolism Exam Questions with Latest Update
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Types of Tests (4) - ANSWER-TSI 
 
Citrate 
 
Carbohydrate Fermentation Test w/ Durham Tubes 
 
MR/VP 
 
TSI Test - ANSWER-Triple Sugar Iron Test (slant) 
 
both a streak and a slant 
 
Citrate test - ANSWER-typically only a streak is done but both a streak and slant may be performed 
 
MR/VP test - ANSWER-Methyl Red 
Voges- Proskauer 
 
TSI Composition - ANSWER-Basic T Soy Agar plus: 
 
1. glucose, lactose, & sucrose. Lac and Suc are 10x the conc. of glucose 
 
2. Phenol red (pH indicator) pH 6...
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BIOD 171 Lab Exam 7 with complete solutions
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What color(s) was the TSI agar slant containing Salmonella? 
red slant, yellow bottom and no gas production. 
Yellow and Black. The agar slant was yellow at the bottom while black on the sloped gradient 
portion of the agar.
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PORTAGE LEARNING BIOD 171 LAB EXAM 1-9
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PORTAGE LEARNING BIOD 171 LAB EXAM 1-9 
 
LAB 1 
 
Answer the following questions 
 
1.	What three elements are used in an autoclave to sterilize equipment? 
 
heat, pressure, and steam 
 
2.	What is the minimum temperature an autoclave must be set at to achieve sterile condition? 
 
125°C 
 
3.	If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your...
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micro lab quiz #3 Question with complete solution 2023
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micro lab quiz #3 Question with complete solution 2023for what bacteria would you use the TSI test 
, proteus vulgaris and shigella flexneri 
 
 
 
why must TSI test observations be made between 18 to 24 hours after inoculation 
because it has to incubate for that amount of time to detect the presence of sugar fermentation, gas production & H2S production 
 
 
 
distinguish between an acid and alkaline slant 
high concentration creates excessive acid formation -- alkaline slant-red slant limited...
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TSI Agar Test Question and answer 100% solved
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TSI Agar Test Question and answer 100% solvedTriple Sugar Iron Test 
What is the name of this test? 
 
 
 
Differentiate between gram-negative rods 
What is the purpose of this test? 
 
 
 
Slant - Acid or Alkaline 
Butt - Acid or Alkaline 
Gas Production - + or - 
Hydrogen Sulfide Production - + or - 
What is the order of the results of this test? 
 
 
 
Phenol Red 
What is the pH indicator for carbohydrate fermentation? 
 
 
 
Ferrous Sulfate 
What is the color indicator for hydrogen sulfide p...
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triple sugar iron test Question and answer 100% solved
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triple sugar iron test Question and answer 100% solvedwhat is the purpose of the triple sugar iron test? 
-the TSI test is designed to differentiate among the different groups or genera of the Enterobacteriaceae, which are all gram-negative bacilli capable of fermenting glucose with the production of acid, and to distinguish the Enterobacteriacase from other gram-negative intestinal bacilli. 
 
 
 
What is the enterobacteriacase? 
-all gram negative bacilli capable of fermenting glucose with the...
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carbohydrate fermentation and TSI agar quiz with complete solution 2023
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carbohydrate fermentation and TSI agar quiz with complete solution 2023name two media that can be used to test for the ability to ferment lactose? 
EMB plate , macConkey plate, phenol red lactose broth,TSI agar slant 
 
 
 
how TSI agar inoculated with what tool? 
stab and streak with an inoculation needle 
 
 
 
which describes the appearance of a surcrose and glucose fermenting organism with TSI agar? 
acid slant and acid butt 
 
 
 
what is often added to media that reacts with sulfur to prod...
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TSI Tests Question with complete solution 2023
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TSI Tests Question with complete solution 2023What does TSI mean? 
Triple Sugar Iron 
 
 
 
What is a TSI (Triple Sugar Iron) Test also referred to as? 
Kligers Iron Test 
 
 
 
What does a TSI Test, test for? 
Bacteria's ability to: 
-Ferment glucose and lactose/sucrose 
-Produce gas 
-Reduce Sulfur 
 
 
 
What are the ingredients of this media? 
Glucose, Sucrose, and Lactose 
 
 
 
What is the ratio of Glucose, Sucrose, and Lactose in this media? 
1:10:10 
 
 
 
What is the sulfur source in a...
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PORTAGE LEARNING BIOD 171 LAB EXAM 1-9
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PORTAGE LEARNING BIOD 171 LAB EXAM 1-9 
 
LAB 1 
 
Answer the following questions 
 
1.	What three elements are used in an autoclave to sterilize equipment? 
 
heat, pressure, and steam 
 
2.	What is the minimum temperature an autoclave must be set at to achieve sterile condition? 
 
125°C 
 
3.	If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your...
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Triple Sugar Iron Agar (TSIA) Question and answer 2023
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Triple Sugar Iron Agar (TSIA) Question and answer 2023What is the Triple Sugar Iron used for? 
The TSI test is used to determine fermentation and sulfur reduction in Gram negative bacteria. 
 
 
 
How many sugars does the medium contain? 
3. lactose, glucose, and sucrose 
 
 
 
What is the pH indicator in the medium? 
phenol red 
 
 
 
What is the sulfur reduction indicator in this medium? 
ferrous oxide () 
 
 
 
What is the sulfur source in this medium? 
sodium thiosulfate 
 
 
 
Sulfur reduc...
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