Tsi agar slant Study guides, Class notes & Summaries

TSI Agar correct answer: *stab and drag *yellow= acid production/ fermentaion of the sugars in the slant/butt. *red= negative *black= H2S production (salmonella or proteus) what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates that the amino acid tryptophan has been degraded into indole. Methyl red test results? correct answer: red= lots of acid (PH of 4) normally yellow=acidic Simmons citrate correct answer: to see i...

Lab 7 Notebook Title: SK07 Secondary Assays for Bacterial Characterization Objective: To profile a bacterial populations based on additional biochemical approaches to better determine growth/metabolic characteristics Procedure: Indole Test (Test #1) 1. Using a sterile look pick an isolated colony from BAP 2. Smear directly onto the reaction area of the slide 3. Examine test area for color change within 20 seconds Indole Test (Test #2) 1. Using a sterile loop pick an isolated colony fro...

bio 171 Lab 7 Notebook. Detailed working, solutions, memo, notes & explanations. Lab 7 Notebook Back to Home Page Title: PL#7 Secondary Assays for Bacterial Characterization Objective: This lab identifies the profile of bacterial populations that are based on additional biochemical approaches to best determine the growth and metabolic characteristics. Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown) tryptophan into indole. Procedure: Test #1 1...

Foods associated with Salmonella outbreak correct answer: eggs, meat, poultry detection and isolation procedure for salmonella correct answer: pre-enrichment selective enrichment streak for isolation confirmation using slants further confirmation using PCR media used for pre-enrichment of salmonella correct answer: LB broth - lactose broth media used for selective enrichment of salmonella correct answer: SC and RV broth media used for isolation of salmonella correct answer: ...

SALMONELLA NOTES QUESTIONS AND ANSWER 2023 GRADED A+ UPDATE Salmonella ANSWER = Genus with over 2200 pathogenic serotypes Serotype classification ANSWER = Based on Kauffman-White system Gastroenteritis ANSWER = Moderate to severe illness caused by Salmonella Transmission ANSWER = Contaminated water and undercooked food, especially chicken Salmonella typhi ANSWER = Causes typhoid fever Salmonella Subgroup I ANSWER = Includes Salmonella typhi and Salmonella paratyphi Suspect Salmonel...

Triple Sugar Iron Agar (TSI) Questions and Answers 100% correct What is the TSI test used to determine? It is used to determine fermentation and sulfur reduction in Gram negative enteric bacteria What are the 3 sugars used in the TSIA test? Sucrose (present in high concentrations) Lactose (present in high concentrations) Glucose (present in low concentrations) K/A H2S+ K/K H2S- K/A H2S- A/A H2S- Control Sucrose Disaccharide Lactos...

Portage Learning BIOD 171 Lab Exams 1-8 Lecture Exams, 100% Accurate Answers provided. Portage Learning BIOD 171 Lab Exams 1-8 Lecture Exams, 100% Accurate Answers provided. 1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam 2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 125°C 3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you c...

BIOD 171 lab exams 1-9 LAB 1 Answer the following questions 1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam 2. What is the minimum tem perature an autoclave must be set at to achieve sterile condition? 125°C 3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. Hot steam is the best choic...

What sugars does triple sugar iron agar contain? correct answer: 1% Lactose 1% Sucrose 0.1% Glucose (Dextrose) What is TSI used for? correct answer: To differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates What is fermented with a red (alkaline) slant and a yellow (acid) deep? (K/A) correct answer: Only glucose What is fermented with a yellow (acid) slant and a yellow (acid) deep? correct answer: Glucose and Lactose and/or Sucrose What is fermen...

what is the purpose of the triple sugar iron test? - correct answer -the TSI test is designed to differentiate among the different groups or genera of the Enterobacteriaceae, which are all gram-negative bacilli capable of fermenting glucose with the production of acid, and to distinguish the Enterobacteriacase from other gram-negative intestinal bacilli. What is the enterobacteriacase? - correct answer -all gram negative bacilli capable of fermenting glucose with the production of acid. wh...