MICROBIOLOGY LAB PRACTICAL #2 REVIEW QUESTIONS WITH COMPLETE SOLUTIONS

TSI Agar correct answer: *stab and drag *yellow= acid production/ fermentaion of the sugars in the slant/butt. *red= negative *black= H2S production (salmonella or proteus) what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates that the amino acid tryptophan has been degraded into indole. Methyl red test results? correct answer: red= lots of acid (PH of 4) normally yellow=acidic Simmons citrate correct answer: to see if a bacterial species can use citrate as their only carbon source for growth. positive=blue negative- green malonate broth test correct answer: to see if a bacterial species can use malonate as their only carbon source for growth. positive=blue (means its Klebsiella) negative=green what does motile mean? correct answer: flagellated microbe being able to swim away from the thin middle line of inoculation TSI AGAR correct answer: Pink/red slant - lactose/sucrose is not fermented and no acid production Black - hydrogen sulfide was produced. Yellow butt - dextrose fermentation and acid production Yellow slant - lactose and sucrose are fermented and acid