Protein purification Study guides, Class notes & Summaries

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 Chapter 1: Overview of the Molecular Biology Geber 1800s: He developed ____ that make ways for molecular biology. From 1700s - 1900s there are huge advancements in ___ (start studying small molecules) 1930s- Understanding of ________ happen 1938s - "Molecular Biology" was written by ______. (First time the word molecular biology actually happen) 1944s - _____ shows DNA was the basis for genes 1953 - ____ and ____ determine...

What is the proteome of the cell? correct answer: the full complex of proteins that can be produced using genetic material and post translational modifications What are some example of post translational modifications? correct answer: phosphorylation (signalling) GPI anchor (PM proteins) O-glycans (nuclear and cytosolic proteins) polyubiquitination (can induce protein degredation) How can you watch the purification of recombinant poly-His tagged GFP? correct answer: use Ni2+ immobili...

Matching A) electrophoresis B) hydrophobic interaction C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) nucleases H) chromophore I) foaming J) high level expression K) 2-mercaptoethanol L) positive charge M) cation exchange N) pI O) chymotrypsin P) C-terminal amino acid Q Sodium dodecyl sulfate 1. One of the reasons the primary structure is important for a protein is that it determines the ______ the molecule adopts...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 What is recombinant DNA technology? - answerThe process of taking a gene from one source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and inserting that DNA into bacteria for further study/manipulation Probes - answerSmall pieces of DNA can be used to determine the presence or absence of an allele, which can indicate a predisposition to a specific disease Microarrays - answerAdvance probe-b...

Everyone who may have contact with biohazard items must be educated about dangers associated with those items True "-Pexy" is Surgical fixation Infectious is medical Waste is regulated by the EPA True "Dermopathy" is a (an) Skin disease The bacteria reproductive process that takes place when a mother cell divides into a daughter cell is called Binary fission Autoclave sterilizer is a ____________ class of device Class 1 Promotes healing prevent...

WGU C001 BIOCHEMISTRY STUDY GUIDE QUESTIONS WITH VERIFIED ANSWERS Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chainto a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatog...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 Chapter 1: Overview of the Molecular Biology Geber 1800s: He developed ____ that make ways for molecular biology. From 1700s - 1900s there are huge advancements in ___ (start studying small molecules) 1930s- Understanding of ________ happen 1938s - "Molecular Biology" was written by ______. (First time the word molecular biology actually happen) 1944s - _____ shows DNA was the basis for genes 1953 - ____ and ____ determine...

QBM Final Exam Questions And Answers With Complete Solutions 2024 What is recombinant DNA technology? - answerThe process of taking a gene from one source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and inserting that DNA into bacteria for further study/manipulation Probes - answerSmall pieces of DNA can be used to determine the presence or absence of an allele, which can indicate a predisposition to a specific disease Microarrays - answerAdvance pro...

_______ use an internal RNA primer so that the end of the eukaryotic chromosome can be replicated. a. Primases b. RNA polymerases c. Telomerases d. DNA polymerases e. Helicases c. Telomerases A transmembrane sensor kinase protein senses an environmental condition outside __________ bacteria or in the periplasm of a/an __________ bacteria. a. Gram-positive; Gram-negative b. Gram-negative; Gram-positive c. prokaryotic; eukaryotic d. prokaryotic; archaean e. Gram-positive; aci...