Tsi agar slant Study guides, Class notes & Summaries

TSI (Triple Sugar Iron) Test 32 Questions with Verified Answers List the tests that are involved with TSI. - CORRECT ANSWER H2S Gas and Carbohydrate Fermentation What is the media for H2S Gas? - CORRECT ANSWER TSI agar What method do you use to place content on the media? - CORRECT ANSWER stab and drag motion what is the substrate for H2S gas? - CORRECT ANSWER cystein What is the product for H2S gas? - CORRECT ANSWER H2S What is the reagent for H2S gas? - CORRECT ANSWER iron in...

Foods associated with Salmonella outbreak correct answer: eggs, meat, poultry detection and isolation procedure for salmonella correct answer: pre-enrichment selective enrichment streak for isolation confirmation using slants further confirmation using PCR media used for pre-enrichment of salmonella correct answer: LB broth - lactose broth media used for selective enrichment of salmonella correct answer: SC and RV broth media used for isolation of salmonella correct answer: ...

What does TSI stand for? correct answer: Triple Sugar Iron How do you inoculate a TSI? correct answer: Stab and Drag What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to identify intestinal bacteria. What does a black medium on SIM agar indicate? correct answer: Positive for sulfur reduction and H₂S production What agent do you add to the indole test and what orga...

TSI correct answer: Triple Sugar Iron Yellow= acid production or fermentation of the sugars in the slant or butt. Gas production= indicated by the agar lifting up or breaking Red= negative or uninoculated Black= H2S production due to FeS precipitation (think salmonella or proteus) The source of Sulfur for H2S production by bacteria is the amino acid Cysteine TSI additional correct answer: Triple Sugar-Iron agar slants. is a differential medium containing 3 sugars called glucose...

Microbiology Lab: KIA/TSI Test 31 Questions with Verified Answers What five things are being tested in a KIA/TSI test? - CORRECT ANSWER 1. Glucose fermentation 2. Lactose fermentation 3. Sulfur reduction 4. Gas production 5. Amino acid degeneration What happens to the color of the tube when glucose is fermented? - CORRECT ANSWER The sugar is fermented and acid drops the pH, this causes the tube to turn yellow When the butt of the slant is yellow but the rest of the slant is pink...

TSI Tests 37 Questions with Verified Answers Red slant/ yellow butt - CORRECT ANSWER Glucose only. Protein used in slant Yellow slant/yellow butt - CORRECT ANSWER -Glucose + -Lactose and sucrose + Red slant/ red butt - CORRECT ANSWER -no fermentation Protein catabolized throughout Red slant only - CORRECT ANSWER Aerobic digestion of protein only No change in slant or butt - CORRECT ANSWER Slow or no growth Yellow slant/red butt - CORRECT ANSWER Not possible Black precipita...

TSI stands for________________________? correct answer: Triple Sugar Iron What is the sulfur source in TSI agar? correct answer: Sodium thiosulfate A red slant and a red butt with TSI agar indicates that the microorganism ________? correct answer: Cannot ferment A red slant and a yellow butt with TSI agar indicates that the microorganism ________________________________? correct answer: Ferments only glucose Why must TSI agar be read, 24 hours after inoculation? correct answer: ...

Microbiology Lab Review Questions with Certified Answers Explain why the body tube of the microscope should not be lowered while you are looking through the ocular lens. to ensure that the slide or lens is not broken For what purpose would you adjust the Iris diaphragm? adjust the amount of light coming through the specimen For what purpose would you adjust the course-adjustment knob? to bring the specimen into view For what purpose would you adjust the fine adjustment knob? to bring the spe...

Biochemical Test - Triple Sugar Iron (TSI) Questions with Answers Tests for... - CORRECT ANSWER carbohydrate fermentation, gas production, & hydrogen sulfide production TSI slant - CORRECT ANSWER contains agar, pH indicator (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, & sodium thiosulfate & ferrous sulfate; as media cools after autoclaving, test tubes placed at slanted angle - provides oxygen-rich surface (aerobic) & oxygen-depleted butt (anaerobic) how the test works - CORRECT...

For what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. Why is there more lactose and sucrose in TSI agar than glucose? correct answer: In order to distinguish bacteria that can only ferment glucose from bacteria that ferment lactose and sucrose. It will also allow for greater acid production. TEST RESULTS: red slant/r...