Exercise 5-21 Triple Sugar Iron Agar Questions With Complete Solutions

TSI stands for________________________? correct answer: Triple Sugar Iron What is the sulfur source in TSI agar? correct answer: Sodium thiosulfate A red slant and a red butt with TSI agar indicates that the microorganism ________? correct answer: Cannot ferment A red slant and a yellow butt with TSI agar indicates that the microorganism ________________________________? correct answer: Ferments only glucose Why must TSI agar be read, 24 hours after inoculation? correct answer: TSI agar runs out of glucose after 12 hours and breaks down amino acids producing ammonia, which raises pH A yellow slant and a yellow butt with TSI agar indicates that the microorganism_________________________________? correct answer: Ferments lactose and/or sucrose A red slant and a black butt indicates with TSI agar indicates that the microorganism is able to_______________________________ and_____________________? correct answer: Ferment glucose and produces hydrogen sulfide The sugars found in TSI agar are______________, _____________, and _________? correct answer: Glucose, lactose, sucrose If a tube of TSI has a yellow slant and butt and the medium is being broken apart, cracked or lifted, what is the microorganism doing in this test tube? ________ and ___________? correct answer: Lactose and/or sucrose fermentation; gas production What is the test indicator in TSI agar? correct answer: Phenol red How much more lactose and sucrose is there than glucose in the TSI agar? correct answer: 10 x more How much glucose is in the TSI agar? correct answer: 0.1% How much lactose and sucrose are there in the TSI agar? correct