Northern blot Study guides, Class notes & Summaries

Central Dogma DNA → RNA → Protein Purines - which bases? - how many rings? Brainpower Read More 0:14 / 0:15 Pyrimidines - which bases? - how many rings? Remember: "Pyrimidine" has a "y" in it; so does "cytosine" and "thymine." Phosphodiester Bond Joins one nucleotide to the next; between the 3rd C of one ribose and the 5th C of the other to create the sugar-phosphate backbone Directionality of DNA (convention) 5' → 3' (but strands are ant...

AAMC full length 2, EXAM Overviews 2023 Update amide linking is peptide on the carboxyl side - ANS-so amide linkage involving the N atom in AMC used to covalently attach flurophore to the peptide what expression gives the amount of light energy (J/photon) that is converted to other forms between the fluorescence excitation and emission events? - ANS-E=hf = hc/lambda, here we see that excitation is at 360 nm but observation is at 440 nm. so you have E = (6.62 x 10^-34)(3x 10^8)x (1/360...

Anticodon - A specialized base triplet at one end of a tRNA molecule that recognizes a particular complementary codon on an mRNA molecule. Bioinformatics - Computational information systems and analytical methods applied to biological problems such as genomic analysis. Comparative Genomics - The study of relationships between genomic sequences of multiple species. Complementary Bases - Two bases that pair together. A is complementary to T and C is complementary to G. Deoxyribonucleic Aci...

Genetics Practice Exam 55 Questions with Verified Answers Which process is represented by the arrow labeled W? A. replication B. translation C. transcription D. RNA splicing - CORRECT ANSWER C Which of the labeled structures contains RNA with a catalytic function? A. 1 B. 2 C. 4 D. 5 E. 2, 3 and 4 - CORRECT ANSWER C Which of the labeled is made of chromatin? A. 1 B. 1 and 4 C. 4 D. 5 - CORRECT ANSWER A Which of the following molecules, NOT represented on this diagram, a...

RNA Polymerase I - ANSWERS-DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - ANSWERS-DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - ANSWERS-DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - ANSWERS-DNA template, product: mRNA Mammalian DNA polymerase α - ANSWERS-DNA template, product: primers HCV RNA Polymerase - ANSWERS-RNA template,...

Key terms and concepts Biology 205 - Genetics Anticodon - answerA specialized base triplet at one end of a tRNA molecule that recognizes a particular complementary codon on an mRNA molecule. Bioinformatics - answerComputational information systems and analytical methods applied to biological problems such as genomic analysis. Comparative Genomics - answerThe study of relationships between genomic sequences of multiple species. Complementary Bases - answerTwo bases that pair together. A i...

Key terms and concepts Biology 205 - Genetics Anticodon - answerA specialized base triplet at one end of a tRNA molecule that recognizes a particular complementary codon on an mRNA molecule. Bioinformatics - answerComputational information systems and analytical methods applied to biological problems such as genomic analysis. Comparative Genomics - answerThe study of relationships between genomic sequences of multiple species. Complementary Bases - answerTwo bases that pair together. A i...

OpenStax Microbiology Test Bank Chapter 12: Modern Applications of Microbial Genetics Chapter 12: Modern Applications of Microbial Genetics * = Correct answer Multiple Choice 1. Which of the following is the definition of biotechnology? A. The alteration of DNA in a cell B. The use of living systems to benefit humankind* C. Using computers to synthesize biomolecules D. Using DNA for solving mathematical problems Difficulty: Easy ASM Standard: 26 2. Which of the following is the defin...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

This document provides a summary of the lecture Single-cell gene expression. Topics that are discussed are: mRNA, mRNA expression, Northern blot, qPCR, RNA sequencing, DNA microarray, gene expression, single-cell gene expression analysis, High-throughput single-cell RNA analysis methods, Low throughput single-cell RNA analysis methods, FISH, smFISH, mRNA localization, Gene expression analysis in living cells and MS2/PP7 systems