- Study guides, Class notes & Summaries

If you want to transfer bacteria between a broth and an agar plate, what would you use? - correct answers an inoculation loop You should wash your hands - correct answers ALL When putting the culture onto the agar in a Petri dish, what do you do with the lid? - correct answers You hold the lid over the agar as much as possible to prevent contamination The techniques needed to prevent the accidental spread of microorganisms beyond the intended working environment - correct answers asepti...

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8 pages

Written in 2025/2026

Grade A+

RealGrades

Contamination is most often due to ... - correct answers failure to adhere to appropriate procedures Differentiation of bacteria into two major groups using the Gram stain is based on ... - correct answers differences in cell wall structure A culture media that contains one or more ingredients that inhibit the growth of certain microbes, but not others, is called a(n) ... - correct answers selective media Microscope slides and any broken glass generated in the laboratory should be ... -...

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5 pages

Written in 2025/2026

Grade A+

RealGrades

TSA - correct answers Tryptic Soy Agar -general bacterial medium BHI - correct answers Brain-Heart Infusion -general bacterial medium PDA - correct answers Potato Dextrose Agar -general fungal medium Microorganisms are - correct answers ubiquitous -most successful life form on earth Importance of Microbes - correct answers-cause disease in plants and animals -be used to produce antibiotics and other pharmaceuticals -used to produce a wide range of food products -used in in...

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16 pages

Written in 2025/2026

Grade A+

RealGrades

aseptic technique - correct answers the method of handling microbes and materials in a way that minimizes contamination inoculation - correct answers process of transferring a microbe from one medium to the next inoculum - correct answers sample being transferred inoculating loop and inoculating needle - correct answers two instruments used to transfer an inoculum isolation streak plate - correct answers widely used method to separate individual bacteria from mixtures colony - cor...

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6 pages

Written in 2025/2026

Grade A+

RealGrades

If a bacterial cluster appears as a square of four cocci, this arrangement is called as _________ - correct answers tetrad A streptococcus arrangement shows __________ - correct answers cocci in chains a chain of round bacteria - correct answers streptococcus irregular group of round bacteria with no specific arrangement - correct answers staphylococcus group of cocci arranged in cluster of 8 - correct answers sarcinae diplobaccilus - correct answers two rod shaped bacteria attach...

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3 pages

Written in 2025/2026

Grade A+

RealGrades

The reagent that must be added to the starch agar plate to confirm hydrolysis is - correct answers Iodine The enzyme that catalyzes the conversion in starch hydrolysis is - correct answers Amylase what color should the starch agar plate become ( after the addition of iodine) if the organism is starch hydrolysis - correct answers negative _ dark positive _ clear what is the indicator used in carbohydrate fermentation test? - correct answers Phenol Red what is the purpose of the Durha...

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3 pages

Written in 2025/2026

Grade A+

RealGrades

Why are agar plates incubated upside-down? - correct answers To keep condensation from forming on the lid and dripping onto the bacteria. Dripping liquid affects colony count and morphology. Why is the inoculating loop sterilized between streaking quadrants? - correct answers To facilitate isolation of microorganisms - form individual CFUs. Also provides accurate enumeration of bacteria. Why can a single colony on a plate be used to start a pure culture? - correct answers A single colony i...

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7 pages

Written in 2025/2026

Grade A+

RealGrades

Base - correct answers Supports the microscope. It is important that when carrying the microscope you have one hand under the base and one wrapped around the arm. Arm - correct answers is important for carrying the microscope. Stage (mechanical stage) - correct answers platform that holds the microscope slide and slide holder . This platform function is to hold an object for viewing. Slide holder - correct answers is the spring loaded clamp and its job is to hold a microscope slide firm...

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10 pages

Written in 2025/2026

Grade A+

RealGrades

What type of media would you use to allow growth of many bacterial species from the sampled environment? - correct answers-TSA (Tryptic soy agar) Where would you incubate a soil sample? - correct answers25 degree celsius incubator Where would you incubate a mouth/touch plate? - correct answers37 degree incubator because it's the human body temp Where would you incubate a sample from the frozen tundra? - correct answersin a fridge thats about 4 degrees celsius??? Resolution definit...

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19 pages

Written in 2025/2026

Grade A+

RealGrades

Bacillis Bacteria - correct answers Rod Shaped, Move easily (tail) Cocci Bacteria - correct answers Coccus, Not mobile. Streptococcus - correct answer Linear Row of coccus Diplococcus - correct answers Two linear cells of coccus Staphylocci - correct answers Cells grouped together of coccus Ubiquity of bacteria - correct answers Most are not pathogens... but actually normal flora and beneficial and essential to higher organisms. Immersion Oil - correct answers Oil forms a con...

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5 pages

Written in 2025/2026

Grade A+

RealGrades

What are the different types of PPE found in the lab? - correct answers gloves and goggles BSL2 - biosafety cabinets BSL3 - respirators, and double gloves BSL4 - hazmat or pos pressure suits What are the different biosafety levels? Which one is the most dangerous? - correct answersBSL1 - least risk/harm, do not typically cause disease BSL2 - may cause disease BSL3 - causes potentially lethal disease BSL4 - causes deadly disease without treatment or vaccine What are some hazards that ...

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9 pages

Written in 2025/2026

Grade A+

RealGrades

Lab coats, gloves, and respirators must be worn as personal protective equipment (PPE) in this type of lab. - correct answers BSL 3 The organisms used in the General Microbiology lab fall under which biosafety levels? - correct answers1 and 2 Test tubes are disposed of in/on the... - correct answers discard cart What is disposed of in the biohazard can? - correct answers Petri plates, and anything contaminated with bacteria Biosafety level used for indigenous microorganisms that can ...

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8 pages

Written in 2025/2026

Grade A+

RealGrades

BSL-1 - correct answers Do not cause disease in healthy individuals Standard practices that are common in all labs BSL-2 - correct answers Moderately hazardous and can cause some mild diseases Lab coats must be warn and gloves and face protection may be used as well BSL-3 - correct answers Can cause serious and lethal disease through respiratory transmission Light Microscopes - correct answers Use light to observe specimen Bright Field Microscopes - correct answers Light microscope...

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2 pages

Written in 2025/2026

Grade A+

RealGrades

what are the three color possibilities for the results on the HE plate and what do they mean? - correct answers yellow/orange= ferments lactose or sucrose with moderate acid production: coliform Blue/green with black precipitate= reduces sulfur and does not ferment lactose or sucrose: salmonella Blue/green= does not ferment lactose/sucrose: shigella what type of clinical problem is the he plate particularly useful for? - correct answersid food poisoning What three test could you us...

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3 pages

Written in 2025/2026

Grade A+

RealGrades

If an organism is being viewed with the 40X objective lens on a standard bright light microscope, how much is the image you see magnified? I.e. what is total magnification? - correct answers(objective lens magnification)*(10) (40X)*(10) = 400X Define the term microorganism - correct answers A living organism that is physically to small to be seen by the naked eye Morphologies - correct answers shapes given to certain bacteria Coccus (Circular) - correct answers small circular colonies ...

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16 pages

Written in 2025/2026

Grade A+

RealGrades

Total Magnification - correct answer stake the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X. 4x - correct answers Scanning lens 10x - correct answers low power lens 40x - correct answers high power dry lens 100x - correct answers High power oil immersion lens. Oil must be used to focus properly. Condenser - correct answers used to stage clamp. adjust both intensity and contrast of light. Aperture lever located on front adjusts contr...

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5 pages

Written in 2025/2026

Grade A+

RealGrades

Aseptic technique includes... (a) sterilization of transfer tools before and after use (b) disinfection of bench tops (c) washing hands (d) all of the above - correct answers(d) all of the above Placing the cap of a broth cultures up on the bench top is acceptable True/False? - correct answers False Why is it important to allow the inoculating loop to cool prior to obtaining the inoculum? - correct answers to avoid frying the cell A pure culture can be prepared from a mixed broth o...

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9 pages

Written in 2025/2026

Grade A+

RealGrades