Microbiology lab exam 2 UTTYLER Sub Advanced Preset Questions With 100% Verified.

what are the three color possibilities for the results on the HE plate and what do they mean? - correct answers yellow/orange= ferments lactose or sucrose with moderate acid production: coliform Blue/green with black precipitate= reduces sulfur and does not ferment lactose or sucrose: salmonella Blue/green= does not ferment lactose/sucrose: shigella what type of clinical problem is the he plate particularly useful for? - correct answersid food poisoning What three test could you use to help id gram positive bacteria - correct answersMsa, bile esculin, hemolysins what are the color possibilities on an emb plate and what do they mean - correct answerspink mucoid= ferments lactose sucrose and little acid prod.: coliform purple/black w or no green metallic sheen= ferments lactose sucrose and high acid prod.: E. coli colorless= does not lactose or sucrose non coliform what is a environmental problem that an emb would help figure out - correct answerssampling areas have been contaminated by sewage what types of bacteria are selected for on mac, emb and he plate - correct answers gram - would you use a mac, emb, or he plate to figure out what you think is staph aureus - correct answers no because staph aureus is a gram + what does the shape of an OF tube accomplish - correct answers if bacteria use oxidative or fermentation pathway to metabolize sugar aerobic= allows air in anaerobe= sealed so air cant diffuse in what do the results of an OF tube test for an obligate aerobe look like - correct answers Yellow what does an obligate anaerobe look like in an OF tube - correct answers Green What does an facultative organism look like in an OF tube - correct answers Yellow What does the citrate test test for - correct answers changes to a basic ph using citrate permease and citritase does the citrate test result show because acids are ultimately produced - correct answers No a basic environment is produced what types of products causes the color change in the citrate test? and what colors are produced - correct answers ammonium phosphate= ammonia + sodium bicarbonate and blue =+ and green=- what blood type is the universal recipient and why - correct answers AB+ and because it has A, B, and Rh antigens what does the enzyme urease do - correct answers hydrolyze urea to ammonia and Co2 Does a negative on the urea test tell you that a patient does not have a uti - correct answers no its not proteus morganella or hellobacter this test helps you id what bacteria it is what are the possible results for the phenol red test and what do they mean - correct answers Yellow with bubbles=ferments prod. acid and gas bubbles A&G: E.coli yellow no bubbles= ferments and prod. acid A: E. faecalis red, orange/pink broth= no ferment Fuchsia no bubbles= degradation of protein product prod. basic environment K. Ketosis what does the phenol red test test for? and what sugars can be tested - correct answers fermentation of carbohydrates/ glucose, sucrose, lactose what type of sugar did we use for the phenol red test - correct answers glucose what does the enzyme tryptophanase do? what reagent is added to tryptophan look for the activity of tryptophanase? - correct answers hydrolyze tryptophan to pyruvate, ammonia and indole kovae's reacts with indole to prod. red color what does a mobility test look like with one positive result and one negative? - correct answerspositive has no line and negative has line whats does the s in sim tube name refer to? - correct answerssulfur