B3201 LAB EXAM 1 2025 Newest With Detailed Verified Answers Success Guaranteed.

Lab coats, gloves, and respirators must be worn as personal protective equipment (PPE) in this type of lab. - correct answers BSL 3 The organisms used in the General Microbiology lab fall under which biosafety levels? - correct answers1 and 2 Test tubes are disposed of in/on the... - correct answers discard cart What is disposed of in the biohazard can? - correct answers Petri plates, and anything contaminated with bacteria Biosafety level used for indigenous microorganisms that can lead to diseases of varying severity in healthy adults. - correct answers BSL 2 True or False? Lab coats are not required when working in the lab. - correct answers False The following people are allowed in the lab... - correct answers students enrolled in the lab The ______ is the horizontal area on the microscope that supports the microscope slide. - correct answers Stage Bright-field microscopes have three lens systems... - correct answers oculars, objectives, and the condenser When focusing the ___ objective, you only adjust the coarse focus adjustment/knob. - correct answers10X _________ is the cut off time for the color changing to occur with the oxidase test and results to be interpreted. This is because oxygen in the environment can create a false positive result if results are read after this amount of time. - correct answers30 seconds Gram positive bacteria stain is the color - correct answers purple True or False? A simple stain utilizes two stains - correct answers False Counter stain for Gram Stain - correct answers safranin When mixed with KOH, Gram negative bacterial cells... - correct answers lyse releasing viscous DNA True or False? The catalase test determines the presence or absence of cytochrome oxidase in organism - correct answers False Gram negative bacteria stain is the color... - correct answersred Spherical morphology of bacteria - correct answer scocci Decolorizer for Gram Stain - correct answers ethanol The Gram stain is a ______ stain - correct answers differential Reagent used to test for catalase enzyme - correct answers Hydrogen Peroxide True or False? The catalase test determines the presence or absence of cytochrome oxidase in a microorganism - correct answers False The reagent used in the coagulase test is - correct answers blood plasma True or False? Staphylococcus aureus is the only Staphylococcus species in your lab reading that gives a positive coagulase result - correct answers True A medium that allows certain bacteria to grow but inhibits other bacteria. - correct answersselective medium A/an _______ medium contains substances that cause some bacteria to take on an appearance that distinguishes it from other bacteria - correct answersdifferential In ________________ tubes, a pH change is indicated by a color change - correct answersphenol red carbohydrate When carbohydrates are fermented in the presence of phenol red (for example in a Phenol red glucose tube) the liquid's color changes from ____ to _______ - correct answersred to yellow The starch hydrolysis test detects the production of.... - correct answersamylase Incomplete breakdown of red blood cells by bacteria that results in a greenish discoloration around colonies - correct answersalpha After incubation, ________ is added to the starch plate to detect starch hydrolysis. - correct answersGrams iodine A/an _________ medium contains substances that cause some bacteria to take on an appearance that distinguish it from other bacteria - correct answersDifferential Sodium chloride in mannitol salt agar allows ______ to grow while inhibiting bacteria that can not tolerate the salt concentration. - correct answersstaphylococci True or False: the catalase test determines the presence or absence of cytochrome oxidase in your organism - correct answersFalse Catalase degrades ______ to water and oxygen - correct answershydrogen peroxide Bile esculin agar is.... - correct answersselective and differential Hemolytic capability of an organism can be demonstrated on... - correct answersSBA Plate Nitrate reagent A and Nitrate reagent B are added to a Nitrate tube that has been incubated. The liquid in the tube turns red. This means... - correct answersnitrate was reduced to nitrite Microorganisms used in this course may be pathogenic for - correct answershumans and animals You should dispose of slides, swabs, and pipettes in the - correct answerssharp container glassware such as flasks, beakers, bottles, test tubes, etc should be placed on the - correct answersdiscard cart Which Biosafety Level is being described? Microorganisms that are not known to cause disease in healthy adults. - correct answersBSL 1 Which Biosafety Level is being described? Indigenous microorganisms that can lead to diseases of varying severity in healthy adults. - correct answersBSL 2 Which Biosafety Level is being described? Indigenous or exotic microorganisms that cause serious or potentially lethal disease through respiratory transmission - correct answersBSL 3 Which Biosafety Level is being described? Microorganisms that are dangerous and exotic with high risk of aerosol transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and the diseases they cause are frequently fatal. - correct answersBSL 4 If you are in Biosafety Level ____, the laboratory must be in a separate building or in an isolated and restricted zone of the building - correct answersBSL 4 _________________ is a general term for living organisms that cannot be seen without a microscope, even though some people include viruses and prions in the group. - correct answersMicroorganisms "Ubiquity of Microorganisms," refers to the concept that microorganisms are _______. However, that's not entirely true. - correct answerseverywhere The only places where microorganisms are not found are places where we intentionally ______ them. - correct answersexclude TSA stands for - correct answersTryptic Soy Agar SBA stands for - correct answersSheep Blood Agar The first thing you should do when you get to your lab table is - correct answersdisinfect the area with lysol is it necessary to allow the loop/needle to cool before transferring an inoculum of bacteria, if so, why? - correct answersyes! if the loop is not cooled down you could kill the bacteria that you are sampling. __________ can destroy vegetative cells and viruses but may not destroy endospores - correct answersdisinfectants SBA is a _______ medium - correct answersdifferential Complete lysis of red blood cells around a colony is known as ______ ________ and results in a clear zone surrounding the colonies. - correct answersbeta hemolysis Partial breakdown of red blood cells producing a greenish discoloration around the colonies is known as ______ ________ - correct answersalpha hemolysis Species of bacteria that do not exhibit any hemolysis of blood display ________ _________ and have no effect on the red blood cells in a blood agar plate. - correct answersGamma hemolysis anerobic - correct answersoxygen required for growth aerobes produce the enzyme _________, to avoid damage - correct answerscatalase bacteria that can not grow in the presence of oxygen - correct answersstrict anaerobes anaerobes that tolerate oxygen - correct answersaerotolerant bacteria bacteria that can grow respiration or fermentation/with or without O2 - correct answersfacultative anaerobes ______ alkaline pH values to _____ at acidic pH values - correct answersred to yellow peptone is known as a - correct answersprotein source ______ ______ is a pH indicator that makes phenol red carbohydrate tubes a type of differential media - correct answersphenol red starch molecules consists of two constituents: - correct answersamylose and amylopectin _________: a straight chain polymer of 200-300 glucose molecules - correct answersamylose _______: a larger branched polymer of glucose - correct answersamylopectin Starch hydrolysis is detected by adding _______ ________ to a starch agar plate - correct answersgrams iodine Iodine complexes with the starch macromolecule and causes the starch agar to turn - correct answers black/brown If the starch in the medium has been degraded by amylase produced by the bacteria, the starch agar adjacent to the bacterial growth will be _______ after the addition of the iodine rather than black/brown - correct answers clear which test is an excellent definitive test for confirming identification of Staphylococcus aureus, since 97% of the strains have proven to be ___________ _________? - correct answers cogulase test; cogulase positive The other two species of Staphylococcus aureus that we use in lab will always be - correct answers coagulase negative Red coloration after the Nitrate A and B reagents OR no color change after the addition of zinc in the Nitrate Reduction test would be a ____________ interpretation - correct answers positive Red coloration after the addition of zinc in a Nitrate Reduction test would indicate a _____ interpretation - correct answers negative Any blue to purple color change within 30 seconds in an Oxidase test would be a _______ interpretation - correct answers positive No color change or blue color within 30 seconds in an oxidase test would be a _________ interpretation - correct answers negative Yellow Color (fermentation) in a Phenol Red Carbohydrate test would be a _________ interpretation - correct answers positive Red color in a Phenol Red Carbohydrate test would be a _________ interpretation - correct answers negative