Micro LAB Exam 1 Actual Exam Questions And Correct Verified Answers.

If you want to transfer bacteria between a broth and an agar plate, what would you use? - correct answers an inoculation loop You should wash your hands - correct answers ALL When putting the culture onto the agar in a Petri dish, what do you do with the lid? - correct answers You hold the lid over the agar as much as possible to prevent contamination The techniques needed to prevent the accidental spread of microorganisms beyond the intended working environment - correct answers aseptic technique In the microbiology lab, culture media is used to grow the bacteria. Liquid media is called - correct answers Broth When you first open a culture tube and before the lid is placed on it, the opening is flamed (run through the Bunsen burner flame). Why? - correct answers To keep bacteria in the air from entering the culture tube and contaminating the culture To keep any bacteria in the air inside the culture tube from leaving it and contaminating the area In the microbiology lab, bacteria are often grown on a gelatinous solid media referred to as an - correct answers Agar What is an inoculation needle used to do? - correct answers Insert bacteria deep into the agar in a culture tube by stabbing If you light the Bunsen burner and the flame is more red and yellow than blue. How can you adjust it? - correct answers Change the oxygen flow by turning the knob at the bottom This is an diagram of an inoculate loop. When you sterilize it, what part/s need to be heated to red hot? - correct answers All of the wire and loop When should you disinfect the lab bench? - correct answers Both before you begin working and after you have completed your work Where are glass culture tubes which are ready for disposal placed? - correct answers In a rack for autoclaving How do you dispose of a slide with bacteria on it? - correct answers Put it in a biohazard bag Place it in disinfectant for at least 30 minutes How should contaminated materials such as plastic petri dishes, plastic inoculation loops, and paper towels be disposed of? - correct answers They go into a biohazard container Before you begin your work in a microbiology lab, what should you make note of? - correct answers The location of fire extinguishers The location of safety showers and eye washes The location of the exits What should be done with a dirty lab coat? - correct answers Leave it in the lab or put it in the lab laundry What should always be worn in a microbiology lab? - correct answers ALL Closed toed shoes Goggles Lab coat What should you do if you spill a bacterial culture? - correct answers Cover it with paper towels, soak the towels with disinfectant, let it set for at least 15 minutes and then put the paper towels in biohazard Which of the following is defined as the ability to distinguish fine detail? - correct answers resolution Which objective lens provides the highest total magnification? - correct answer soil immersion The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used. - correct answers multiplying Which of the image quality factors is usually expressed in a number such as 40x? - correct answersmagnification You have successfully focused on an object with the 10x and 40x objective lenses. You have rotated the 100x objective lens into position but are unable to obtain a sharp image by rotating the fine focus adjustment knob. Which of the following is most likely to correct this problem? - correct answers Add a drop of immersion oil before rotating the 100x lens into position. All of the following that are factors affecting the clarity of a microscopic image EXCEPT - correct answers slide material This will contain a lens at the top of the body tube - correct answers Eyepiece/ocular This is used to move the stage upward or downward - correct answers Coarse focus knob Platform that supports a microscope slide - correct answers stage Which part of the microscope will be used first to adjust the focus when starting with the lowest power lens? - correct answers coarse focus When preparing a wet mount specimen for viewing, the specimen should be covered with - correct answers a coverslip. Select all of the following that are major approaches to studying microorganisms. (Select all that apply) - correct answers genetic microscopic biochemical Select all of the following that are NOT microscopic organisms. - correct answers plants mushrooms vertebrates The magnification of the ocular lens is - correct answers10x. When the scanning (4x) objective is used with a 10x ocular, the total magnification will be - correct answers40X Staining cells such that their color is different from the background enhances their - correct answers contrast. After focusing on an object with the low power objective lens, you are trying to bring it into focus under the 40x objective lens, but the field of view is very dark. Which of the following may remedy this problem? - correct answers Increase the light intensity. A change in what component of microscopy influences the apparent size of the objects being viewed? - correct answers magnification This is the circular area seen through the eyepiece - correct answers Field of view This holds the microscope slide in place - correct answers Stage/slide clip The objective lenses of the compound light microscope are attached to the - correct answers rotating nosepiece. If leaving an objective lens over the stage when storing the microscope, which objective lens should be placed over the stage? - correct answers scanning 4X Which of the following approaches to the study of microorganisms enables direct viewing of microbial cells? - correct answersmicroscopic How is an inoculating loop or needle sterilized prior to use? - correct answers holding in the hottest part of the Bunsen burner flame until red-hot How is air contamination prevented when an inoculating loop is used to introduce or remove organisms from an agar plate? - correct answersThe plate lid is kept closed or lifted only slightly when inoculating. Microorganisms are found in nearly every environment and are said to be - correct answers ubiquitous. Which statement correctly describes the level of contamination on different objects? - correct answers Different objects will vary in their level of contamination. What is the correct way to open an agar plate to either remove a sample or to inoculate? - correct answers Open lid slightly on one side, in a clamshell fashion. Can you be certain that you made a successful transfer of a particular organism if your sterile broth is turbid after incubation? - correct answers No, the broth will be turbid if it is correctly inoculated, but also if it was contaminated during the inoculation procedure. A glass tube of solid medium with an angled surface is referred to as a (an) _______. - correct answers Slant Why is 35-37 degrees Celsius a standard incubation temperature range for many lab exercises involving medically important bacteria? - correct answers This range is close to human body temperature, a temperature preferred by many of these organisms. Petri dishes should be incubated with the lid side up. - correct answers false