MIC 206

what are the Characteristics of a virus? correct answer: 1 Obligate intracellular parasites 2 Small - 20 to 200 nm 3 Acellular 4 Made of nucleic acid surrounded by protein coat 5 Active within cells and inactive when extracellular 6 Uses cellular resources for multiplication 7 Lack metabolic processes 8 Lack translational machinery 9 Do not independently fulfil characteristics of life what are the Functions of the Viral Capsid/Envelope? correct answer: Protects nucleic acids and Fac...

Simmons citrate test: enzyme correct answer: citrate permease Simmons citrate test: pH indicator correct answer: Brothymol blue Results of a positive Simmons citrate test correct answer: Turns blue can use sodium citrate as sole carbon source Results of a negative Simmons citrate test correct answer: Stays green color --> pH doesn't change doesn't contain the enzyme Malonate test: enzyme correct answer: Succinate dehydrogenase What does the Malonate test do? correct an...

Membrane Filter Method correct answer: Selective and differential Media: Fuschin sulfiote indicator to identify lactose fermenters will appear dark red in color All non lactose fermenters will appear colorless Calculate the coliforms CFU per 100ml Countable colonies for membrane filter method correct answer: 20-200 Mold is correct answer: Eukaryotic Aerobic Cell wall has chitin Chemoheterotrophs Filamentous Sporangia Major divisions are based in their sexual reproductive ...

Types of media correct answer: complex, defined, selective, differential Complex media correct answer: Unknown chemical makeup Wide range of growth Defined media correct answer: Known chemical makeup Low range of growth Selective media correct answer: Has a component added that will inhibit or prevent growth of certain of certain types of bacteria or will promote growth of desired species Differential media correct answer: Component is added to distinguish between different ty...

growth of microbes correct answer: increase in cell number 3 growth paterns correct answer: discrete colony, dispersed cells, complex biofilms discrete colony correct answer: aggregation of cells visible on surface of solid media dispersed cells correct answer: single cells suspended in liquid media complex biofilm correct answer: community of one or more species of bacteria doubling time correct answer: the time required for a population to double in size logarithmic gro...

Complex Media correct answer: exact chemical concentration of the media is unknown, wide range of growth Defined Media correct answer: known chemical concentration of the media Selective Media correct answer: media with an inhibiting/promoting component that affects the growth of bacteria species Differential Media correct answer: media with an added component that allows for distinguishing of between different types of bacteria based on an observable trait Aseptic Technique corr...

Major classes of microorganisms correct answer: Virus, Algae, Fungi, Protozoa, Bacteria Bacteria: Prokarote v Eukaryote correct answer: Prokaryote. Only one. Eveything else is a eukarote Compare and contrast prokaryotic cells and eukaryotic cells correct answer: - E have NUCLEUS, P do not (NUCLEOID only). - E have INTERNAL MEMBRANE BOUND ORGANELLES, P do not - Both have CELL MEMBRANE - Both have RIBOSOMES, but E have larger ribosomes. - E use MITOSIS, MEIOSIS, P use BINARY FISSION. ...

what types of nutrients are required for a microorganism to grow correct answer: water, energy source, carbon source, nitrogen, minerals, vitamins Chemotrophs correct answer: break chemical bonds to derive energy Phototrophs correct answer: energy derived from sunlight via photosynthetic pigments hetrotroph correct answer: obtain organic carbon (carbs, protein, fats, DNA, amino acids) autotroph correct answer: obtain carbon by fixing CO2 states of media correct answer: liqui...

What is the autoclave used for? correct answer: Sterilization What is the correct temperature, pressure, and time frame when using the autoclave? correct answer: 121°C 15psi 15 minutes (increased volume = increased time) Define complex media correct answer: Media where the exact chemical makeup is UNKNOWN and they have a WIDE range of growth Define defined media correct answer: Media where the exact chemical makeup is KNOWN and they have a NARROW range of growth What is a se...

Antibiotics are produced by all of the following except: bacteria fungi viruses chemical synthesis correct answer: viruses Antibiotics that are produced by many different types of bacteria are called broad spectrum antibiotics correct answer: false In the Kirby-Bauer test, any visible zone of inhibition around the antibiotic disk indicates that the bacterium is sensitive to that antibiotic. correct answer: false The Minimum Inhibitory Concentration (MIC): correct answer: is the ...