AAB MT (Hematology)

What is hemoglobin composed of? - ANSWER 4 heme, 4 iron, 4 globins What disorder does a defect in heme synthesis lead to? - ANSWER Porphyria Porphyria - ANSWER Deficiency in the enzymes of the porphyrin pathway leads to insufficient production of heme. The principal problem in these deficiencies is the accumulation of porphyrins, the heme precursors, which are toxic to tissue in high concentrations. What is methemoglobin? - ANSWER Methemoglobin is a form of hemoglobin that contains fe...

What are the functions of proteins? - ANSWER -Transportation of metabolic substances. -Part of the cell membranes. -Part of hormones and receptors. -Components of Enzymes. Proteins are composed of: - ANSWER -Carbon -Oxygen -Hydrogen -Nitrogen (differentiates proteins from carbohydrates & lipids). -Sulfur *Proteins have a high molecular weight, making them good immunogens. What proteins are soluble? - ANSWER Albumin- Soluble in water Globulins- Soluble in dilute salt solutions ...

Which of the four elements in proteins differentiated this class of substances from carbohydrates and lipids? - ANSWER Nitrogen Which of the proteins is soluble in water? - ANSWER Albumin How are amino acids joined together to form a chainlike structure? - ANSWER Peptide bonding Proteins may become denatured when subjected to mechanical agitation, heat, or chemical treatment. What does denaturation of proteins refer to? - ANSWER Alteration of tertiary structure Which aminoacidopathy ...

What term describes the morphology of organisms that vary in shape and size? - ANSWER Pleomorphic Capsules are the basis of which test? - ANSWER Serotyping A mucoid colony on blood agar medium indicates that which of the following characteristics may be present? - ANSWER Organism has a capsule. What are dark staining granules called? - ANSWER Metachromatic Which of the following organisms are non-motile? - ANSWER Shigella and Klebsiella In the bacterial growth cycle, growth ceases becau...

What are four types of centrifuges commonly used in the clinical lab? - ANSWER Tabletop, Micro, High-Speed, and Ultra. What does the daily maintenance of a centrifuge commonly consist of? - ANSWER Cleaned with 10% bleach What does the periodic maintenance of a centrifuge commonly consist of? - ANSWER Speed check by tachometer What is the function of a centrifuge in the clinical lab? - ANSWER To separate substances based on density by centrifugal forces. What is the RCF formula? - ANS...

Which of the following is not an external defense mechanism of natural immunity that provides the human body with a defense against invading microorganisms? - ANSWER Bursa of Fabricius All of the following are external defense mechanisms of natural immunity that provides the human body with a defense against invading microorganisms - ANSWER Skin, mucus, and body fluids Having an elevated temperature during an infection is an example of what type of immunity? - ANSWER Innate immunity ...

Most common analytical error - ANSWER not checking specimen's integrity most common post-analytical error - ANSWER clerical error how often a procedure be reviewed: - ANSWER every 2 years before inspection thermometers must be checked against NIST thermometer - ANSWER annually following equipment maintenance, when is QC suggested? - ANSWER After any preventative maintenance according to CLIA who is responsible for processing, testing, and reporting results? - ANSWER testing personnel ...

Interferes w/ lymphocyte cell histogram - ANSWER NRBC Interferes w/ lymphocyte cell histogram - ANSWER Malaria Interferes w/ lymphocyte cell histogram - ANSWER Cryoglobulins Interferes w/ lymphocyte cell histogram - ANSWER Sickle cell anemia Interferes w/ lymphocyte cell histogram - ANSWER Bernnard Soulier syndrome Interferes w/ lymphocyte cell histogram - ANSWER Cold agglutinans Interferes w/ monocluear cell histogram - ANSWER Blasts Interferes w/ monocluear cell histogram - ANSWER Lymph...

What agency approves new test methodologies, instruments, and reagents before they are available for purchase by consumers? - ANSWER FDA How often should biological safety hoods be inspected? - ANSWER Annually What is the minimum number of standards required for a standard curve? - ANSWER 3 What is the maximum magnification using a brightfield microscope? - ANSWER 1000X What is the most accurate pipette for reconstituting control material? - ANSWER Glass volumetric pipettes Which unit o...

This DNA amplification method amplifies the nucleic acid used as a probe? - ANSWER Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - ANSWER DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - ANSWER Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - ANSWER Better specificity How does Ligase Chain Reaction differ from Polymerase Ch...