BOC Study Guide Histotechnology 2nd
ed.- Fixation (HT) Exam Questions and
Answers 100% Solved
An example of an additive fixative is one that contains: - ✔✔picric acid
Microscopic examination of an H&E stained section fixed in formalin shows
marked nuclear bubbling. One most often sees this artifact if the specimen
is processed following: - ✔✔incomplete fixation
Microscopic evalution of H&E stained sections from a surgically removed
small bowl specimen shows an absence of much of the epithelium in
otherwise normal tissue. This most likely resulted from: - ✔✔delayed
fixation
When compared with tissue fixed in formalin, tissue fixed in zinc-formalin
will show: - ✔✔superior nuclear detail
A certain project requires a fixative that contains acetic acid yet stabilizes
erythrocyte membranes. One fixative that could be used is: -
✔✔Hollande solution
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A specimen of kidney must be shipped to another city for
immunofluorescence studies. The specimen should be placed in: -
✔✔Michel Solution
When the microwave oven is used for fixation, the most critical factor is the:
- ✔✔control of temperature
To adequately remove the calcium from a specimen containing areas of
microcalcification, the tissue should be fixed in: - ✔✔Hollande solution
Which of the following fixatives contains copper acetate? - ✔✔Hollande
A specimen is submitted with the statement that it was fixed in formalin.
Microscopic sections show marked lysis of erythrocytes. This indicates that
the fixative most likely was: - ✔✔acidified with acetic acid
Fixatives are classified as additive because of the: - ✔✔addition, or
binding of the fixative to tissue proteins.
Kidney biopsy tissue has been fixed in phosphate-buffered glutaraldehyde
for 2 hours and then placed in phosphate buffer solution. If a portion of this
tisssue is processed for light microscopy, sections would most likely show:
- ✔✔nonspecific PAS staining
Uric acid crystals are preserved ONLY when tissue is fixed in: -
✔✔absolute alcohol