BIOL 351- PreLab 1-15 [TAMU]-
Questions with Solved Solutions.
(1) Students will receive a safety violation for all of the following EXCEPT: - Answer Getting out
your phone or electronic device one or two steps after exiting the lab room
(1) If you are absent, what should you do? Choose all that apply. - Answer 1. Complete the Lab
Make-up Request Form within 48 hours
2. If I know about my absence in advance, I need to contact Lacy at least 72 hours before the
absence
(1) True or False - Assignments will only be reopened only if you have a note that excuses you
for the entire time the assignment is available. - Answer True
(1) What is a viable excuse for missing lab? Choose all that apply. - Answer 1. Illness
2. Professional school interview
3. Illness or death of an immediate family member
(1) If you don't agree with a grade you receive or how a question was graded, what is the proper
protocol as listed in the syllabus? - Answer 1. E-mail the TA and include the question that is
being challenged along with an explanation
2. If the TA is not willing to change the grade, e-mail Lacy the grade challenge and include the
TA's response. The grade challenge will then be review by the Lab Director.
(1) What is required if you have technical problems during an online assignment? - Answer 1.
Include a screenshot of the problem in the e-mail
2. E-mail before the due date/time
3. Include the course and section number when e-mailing
(2) Where would you expect to find a saprophyte organism? - Answer In soil
(2) Which of the following things below would result in a pure culture? - Answer A completely
isolated colony growing on a plate
(2) Microorganims are ubiquitous. This means they would be found in which of the following
places?
(a) Soil
(b) The body
, (c) On an aluminum railing
(d) a and b - Answer All of the above
(2) Some bacteria can lyse (break down) red blood cells in a process called hemolysis. Match the
following descriptions of hemolysis with the correct outcome on a blood agar plate:
α hemolysis
β hemolysis
ϒ hemolysis - Answer 1. There will be a greenish colored zone around the bacterial growth due
to incomplete lysis of the red blood cells.
2. The blood agar plate will show clear around the bacterial growth due to complete hemolysis
of the red blood cells
3. The blood agar plate will show no hemolysis
(3) Which of the following things is a safe practice when transferring cultures? - Answer Hold
the inoculating loop like a pencil
(3) The handles of inoculating loops can get very hot. How hot can your inoculating loop get if
you leave it in the incinerator for a really long time? - Answer close to 800*
(3) When a cap is removed from a tube in the transfer process, where does that cap go? -
Answer It is held by the pinky finger of your loop hand
(3) What is the appropriate time to flame your inoculating loop when transferring your culture -
Answer Both before and after you transfer the bacteria
(3) In your epi-tubes you are transferring continually more dilute samples from tube to tube.
According to your spread plate protocol, which of the following represents the order of tubes
from most concentrated to most dilute? - Answer Tube order: A,B,C,D
(3) Which of the following morphology terms would describe a tiny pinpoint colony? - Answer
punctiform
(3) Match the media type below with its intended purpose?
Plates
Agar Slants
Broths - Answer 1. Used to isolate species
2. Used when you want to grow a culture to store in the refrigerator
3. Used to grow cultures
Questions with Solved Solutions.
(1) Students will receive a safety violation for all of the following EXCEPT: - Answer Getting out
your phone or electronic device one or two steps after exiting the lab room
(1) If you are absent, what should you do? Choose all that apply. - Answer 1. Complete the Lab
Make-up Request Form within 48 hours
2. If I know about my absence in advance, I need to contact Lacy at least 72 hours before the
absence
(1) True or False - Assignments will only be reopened only if you have a note that excuses you
for the entire time the assignment is available. - Answer True
(1) What is a viable excuse for missing lab? Choose all that apply. - Answer 1. Illness
2. Professional school interview
3. Illness or death of an immediate family member
(1) If you don't agree with a grade you receive or how a question was graded, what is the proper
protocol as listed in the syllabus? - Answer 1. E-mail the TA and include the question that is
being challenged along with an explanation
2. If the TA is not willing to change the grade, e-mail Lacy the grade challenge and include the
TA's response. The grade challenge will then be review by the Lab Director.
(1) What is required if you have technical problems during an online assignment? - Answer 1.
Include a screenshot of the problem in the e-mail
2. E-mail before the due date/time
3. Include the course and section number when e-mailing
(2) Where would you expect to find a saprophyte organism? - Answer In soil
(2) Which of the following things below would result in a pure culture? - Answer A completely
isolated colony growing on a plate
(2) Microorganims are ubiquitous. This means they would be found in which of the following
places?
(a) Soil
(b) The body
, (c) On an aluminum railing
(d) a and b - Answer All of the above
(2) Some bacteria can lyse (break down) red blood cells in a process called hemolysis. Match the
following descriptions of hemolysis with the correct outcome on a blood agar plate:
α hemolysis
β hemolysis
ϒ hemolysis - Answer 1. There will be a greenish colored zone around the bacterial growth due
to incomplete lysis of the red blood cells.
2. The blood agar plate will show clear around the bacterial growth due to complete hemolysis
of the red blood cells
3. The blood agar plate will show no hemolysis
(3) Which of the following things is a safe practice when transferring cultures? - Answer Hold
the inoculating loop like a pencil
(3) The handles of inoculating loops can get very hot. How hot can your inoculating loop get if
you leave it in the incinerator for a really long time? - Answer close to 800*
(3) When a cap is removed from a tube in the transfer process, where does that cap go? -
Answer It is held by the pinky finger of your loop hand
(3) What is the appropriate time to flame your inoculating loop when transferring your culture -
Answer Both before and after you transfer the bacteria
(3) In your epi-tubes you are transferring continually more dilute samples from tube to tube.
According to your spread plate protocol, which of the following represents the order of tubes
from most concentrated to most dilute? - Answer Tube order: A,B,C,D
(3) Which of the following morphology terms would describe a tiny pinpoint colony? - Answer
punctiform
(3) Match the media type below with its intended purpose?
Plates
Agar Slants
Broths - Answer 1. Used to isolate species
2. Used when you want to grow a culture to store in the refrigerator
3. Used to grow cultures
