ASCP CG FINAL PAPER 2026 FULL Q&A
STUDY GUIDE GRADED A+
◉CLIA 1988- (Clinical Laboratory Improvement Amendments)
stipulates that equipment must be recalibrated __ ? Answer: Every 6
months (Standard 493.1217)
◉What type of stained chromosome would produce a better
computer image? Answer: Lightly stained chromosomes (best
contrast and can be digitally edited easier)
◉What is the nomenclature on a patient with down syndrome
referred for a hematologic disorder where only trisomy 21 was
found? Answer: 47,XY,+21c[20]
◉24 hours after culture initiation, a single dish from an amniotic
fluid turns cloudy. Which cultures should be disposed? Answer: The
single cloudy dish only
◉What is used to reduce mycoplasma contamination? Answer: BM
cycline
,◉CLIA '88 stipulates that equipment must be recalibrated ____ ?
Answer: After a major repair to ensure still operational
◉How should caustic and corrosive chemicals be stored? Answer:
Both should be stored in a nonflammable cabinet
◉What factors would contribute to ALL cultures failing in a CO2
incubator? Answer: Temperature
CO2 level
Humidity level
◉What is the modal number for a diploid karyotype and an
additional CBG negative marker chromosome? Answer: 46
CBG = c-bands achieved with barium with Giemsa stain
CBG negative referrs to not having a centromere, which is crucial to
being included in the modal number, so this extra fragment does not
have a centromere and is not counted towards the total
◉What is included when recording the modal number? Answer: Mar
- a marker chromosome is included because it has a centromere
◉What is the BEST way to avoid producing ghost-like, pale
chromosomes while staining? Answer: Decrease trypsin time
, ◉In order to ensure successful harvest, each new batch of hypotonic
solution should be evaluated ____. Answer: By comparing new
hypotonic solution to that already in use
◉Which cell type would you want to use for long term serial
cytogenetic studies? Answer: Fibroblast cells
◉What is not a necessary (must be added) ingredient which is
added to modified medium? Answer: Antibiotics
◉What chemical is used for cryopreservation? Answer: Dimethyl
sulfoxide (DMSO)
◉You are analyzing a clinical case and discover a small extra marker
with satellites. What staining technique would be most useful in
determining the chromosomal origin of this marker? Answer: NOR
◉When using a fluorescein dye (yellow-green) labeled probe during
the FISH procedure, which counterstain should be used? Answer:
Propidium iodide (red)
◉A bone marrow sample collected in transport medium is leaking
into the sealed plastic bag in which it was transported. The
STUDY GUIDE GRADED A+
◉CLIA 1988- (Clinical Laboratory Improvement Amendments)
stipulates that equipment must be recalibrated __ ? Answer: Every 6
months (Standard 493.1217)
◉What type of stained chromosome would produce a better
computer image? Answer: Lightly stained chromosomes (best
contrast and can be digitally edited easier)
◉What is the nomenclature on a patient with down syndrome
referred for a hematologic disorder where only trisomy 21 was
found? Answer: 47,XY,+21c[20]
◉24 hours after culture initiation, a single dish from an amniotic
fluid turns cloudy. Which cultures should be disposed? Answer: The
single cloudy dish only
◉What is used to reduce mycoplasma contamination? Answer: BM
cycline
,◉CLIA '88 stipulates that equipment must be recalibrated ____ ?
Answer: After a major repair to ensure still operational
◉How should caustic and corrosive chemicals be stored? Answer:
Both should be stored in a nonflammable cabinet
◉What factors would contribute to ALL cultures failing in a CO2
incubator? Answer: Temperature
CO2 level
Humidity level
◉What is the modal number for a diploid karyotype and an
additional CBG negative marker chromosome? Answer: 46
CBG = c-bands achieved with barium with Giemsa stain
CBG negative referrs to not having a centromere, which is crucial to
being included in the modal number, so this extra fragment does not
have a centromere and is not counted towards the total
◉What is included when recording the modal number? Answer: Mar
- a marker chromosome is included because it has a centromere
◉What is the BEST way to avoid producing ghost-like, pale
chromosomes while staining? Answer: Decrease trypsin time
, ◉In order to ensure successful harvest, each new batch of hypotonic
solution should be evaluated ____. Answer: By comparing new
hypotonic solution to that already in use
◉Which cell type would you want to use for long term serial
cytogenetic studies? Answer: Fibroblast cells
◉What is not a necessary (must be added) ingredient which is
added to modified medium? Answer: Antibiotics
◉What chemical is used for cryopreservation? Answer: Dimethyl
sulfoxide (DMSO)
◉You are analyzing a clinical case and discover a small extra marker
with satellites. What staining technique would be most useful in
determining the chromosomal origin of this marker? Answer: NOR
◉When using a fluorescein dye (yellow-green) labeled probe during
the FISH procedure, which counterstain should be used? Answer:
Propidium iodide (red)
◉A bone marrow sample collected in transport medium is leaking
into the sealed plastic bag in which it was transported. The
