Affinity chromatography Study guides, Class notes & Summaries

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2023/2024 COMPLETE SOLUTIONS LATEST 2023/2024 Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Use d in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solu...

Test Bank - Lehninger Principles of Biochemistry 8th Edition by David L. Nelson - Complete, Elaborated and Latest Test Bank. ALL Chapters (1-82) Included and Updated for 2023 #BiochemistryFoundations #ProteinStructure #EnzymeFunction #NucleicAcids #LipidsAndMembranes #MetabolismIntro #Glycolysis #CitricAcidCycle #OxidativePhosphorylation #Photosynthesis #AminoAcidBiosynthesis #GeneExpression #BiochemistryTestBank #Bioenergetics #BiochemicalSignaling Chapter: 1. The Founda...

What are the benefits of having smaller particles in HPLC? - correct answers high efficiency, higher plate number, higher pressure, shorter run time, better resolution due to uniform flow (reduced A-term, multiple pathway) what is plate height? - correct answers length of column required for 1 equilibration of solute between mobile and stationary phases What are the drawbacks of having smaller particles in HPLC? - correct answers lower detection limit, resistance to solvent flow, increased...

History of chromatography (ch 23) - correct answers -Greek word "chromatus" for color -Tswett (1903) first separated plant pigments Applications - correct answers Breast milk, water analysis, urinalysis for drug doping Goals of separation - correct answers Recovery of product, purity of product Type, SP, MP - correct answers 1. Adsorption: one polar substance sticks to another polar substance. Solid SP, gas or liquid MP 2. Partition: dissolving of 2 similar liquids. Liquid SP, gas o...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution....

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Goals of Separation - correct answers recovery of product, purity of product Types of Chromatography - correct answers Adsorption - SP: solid, MP: gas/liquid Partitioning - SP: liquid, MP: gas/liquid Ion-Exchange - SP: bonded anion/cation, MP: liquid Molecular Exclusion - SP: has pores, MP: liquid Affinity - SP: antigen/antibody interactions, MP: liquid Electrophoretic - no SP Solvent Extractions based on - correct answers Some solvents are not miscible; "like dissolves like"; less ...