Gel electrophoresis Study guides, Class notes & Summaries

Restriction Enzyme (Restriction Endonuclease) - Answer-An enzyme produced chiefly by certain bacteria, having the property of cleaving/cutting/digesting DNA molecules at or near a specific sequence of bases. Spectrophotometer - Answer-Used to measure protein or DNA concentration; an apparatus for measuring the intensity of light in a part of the spectrum, especially as transmitted or emitted by particular substances. Horizontal Gel Electrophoresis Box - Answer-Used to run DNA agarose gels....

MCB EXAM 1 QUESTIONS & ANSWERS 2023/2024 How large is our genome? - ANSWER-3200 million bp How many base pairs does the genome contain? - ANSWER-3 × 10^9 base pairs. How long is the genome when fully extended? - ANSWER-20,000 phone books long What is Chromatin? - ANSWER-the fibrous complex of eukaryotic DNA and histone proteins. Which are the main Histones? - ANSWER-H1, H2A, H2B, H3, and H4. What's the charge (polarity) of Histones? - ANSWER-Positive What amino acid resi...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample...

Describe DNA structure - Answer-DNA forms a double stranded helix and adenine pairs with thymine, and cytosine pairs with guanine What pair forms hydrogen bonds and how many - Answer-thymine/adenine form 2 bonds guanine/cytosine form 3 bonds Explain "the central dogma" - Answer-DNA turning into RNA turning into Protein What is transcription - Answer-Copying of DNA code to RNA, one nucleotide at a time so it is complementary to the DNA What is translation - Answer-RNA gets translated int...

what are two factors that contribute to phenotype? - Answer-genotype and environment species - Answer-individuals that are able to exchange genetic material gene pool - Answer-all alleles present in individuals of that species populations - Answer-interbreeding groups of organisms of the same species living in the same geographical area what are two main sources that lead to genetic variation? - Answer-mutation and recombination what can somatic mutations lead to? - Answer-cancer, disease ...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

BACE Practical Review (2023/2024) Graded A Restriction Enzyme (Restriction Endonuclease) An enzyme produced chiefly by certain bacteria, having the property of cleaving/cutting/digesting DNA molecules at or near a specific sequence of bases. Spectrophotometer Used to measure protein or DNA concentration; an apparatus for measuring the intensity of light in a part of the spectrum, especially as transmitted or emitted by particular substances. Horizontal Gel Electrophoresis Box Used to run DNA a...

Test Bank for Prescott's Microbiology 11th Edition by Joanne Willey, Kathleen Sandman & Dorothy Wood 9781260211887 Chapter 1-43 | Complete Guide A+. Prescott's Microbiology, 11e (Willey) Chapter 1 The Evolution of Microorganisms and Microbiology 1) Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. Answer: FALSE Topic: Taxonomy of Microorganisms Bloom's/Accessibility: 2. Understand / Keyboard Navigation ASM Topic: Module 01 Evolution ASM Obje...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution....

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...