Dna polymerases Study guides, Class notes & Summaries

MCB4304 Exam 2 Final Questions & Answers 2024/2025 When trying to solve a protein structure, why is it necessary to grow protein crystals prior to X-ray-crystallography? - ANSWERSthe proteins must all be oriented in the same manner in the unit cell. The helix-turn-helix DNA binding motif is a feature exclusive to prokaryotes. - ANSWERSfalse Helix-turn-helix DNA binding motifs exclusively interact with DNA base pairs. - ANSWERSfalse DNA binding proteins can interact with the DNA str...

MMSC490 Exam 2 Questions with 100% Correct During E. coli DNA replication, all newly synthesized or nascent DNA fragments must have one of the following characteristics a. a 5' hydroxyl and 3' triphosphate b. a 5' triphosphate and 3' monophosphate c. 5' monophosphate and 3' hydroxyl d. 5' triphosphate and 3' hydroxyl - Answer ️️ -d. 5' triphosphate and 3' hydroxyl Among the 3 DNA polymerases in E. coli, DNA polymerases I and III differ in a number of properties that make DN...

The structure of a strand of DNA. - it is a double helix, like a twisted ladder. the ladder rings are nucleotide subunits and they have a covalent bond to the sugar phosphate backbone (made of deoxyribose and a phosphate group) What are the nucleotide subunits? - adenine, guanine, cytosine, and thymine How are the subunits held together? - through a hydrogen bond What are the nucleotide subunits compliments? - Adenine and thymine cytosine and guanine How does DNA replicate? - the parent s...

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...

Nucleic Acid grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide double stranded DNA most energetically favorable state for DNA Purines double-ring structure Adenine (A) + Guanine (G) Pyrimidine single-ring structure Uracil (U)+ Cytosine Messenger RNA (mRNA) -template for all protein synthesis -encode all information necessary to produce proteins Heterogeneous nuclear RNA primary product o...

Nucleic Acid grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide double stranded DNA most energetically favorable state for DNA Purines double-ring structure Adenine (A) + Guanine (G) Pyrimidine single-ring structure Uracil (U)+ Cytosine Messenger RNA (mRNA) -template for all protein synthesis -encode all information necessary to produce proteins Heterogeneous nuclear RNA primary product o...

The sequence of one strand of DNA is shown below. Which of the following is the sequence of its complementary strand? 3′ ATTTGGCC 5′ 5' TAAACCGG 3' DNA polymerases are enzymes that build DNA. They catalyze ____ reactions leading to the formation of ____ bonds. condensation; phosphodiester A bacterium is infected with an experimentally constructed bacteriophage composed of the T2 phage protein coat and T4 phage DNA. The new phages produced would have -------. T4 protein and T4 DNA Which ...

Test Bank for Huether and McCance's Understanding Pathophysiology, Canadian Edition, 2nd Edition by Kelly Power, Stephanie Zettel, , Mohamed Toufic El-Hussein Table of Contents PART ONE: BASIC CONCEPTS OF PATHOPHYSIOLOGY Unit 1: The Cell 1. Cellular Biology 2. Genes and Genetic Diseases 3. Epigenetics and Disease 4. Altered Cellular and Tissue Biology 5. Fluids and Electrolytes, Acids and Bases Unit 2: Mechanisms of Self-Defense 6. Innate Immunity: Inflammation and Wound Healing ...

Similarities between eukaryotic and prokaryotic cells 1) Semi-conservative 2) Bidirectional Prokaryotic cells 1) Circular DNA 2) Single origin of replication Eukaryotic cells 1) Linear DNA 2) Multiple origins of replication New strands are always synthesized from: 5' --> 3' end of each strand Template strand is read: 3' --> 5' Chain extension occurs: by adding a dNTP to free 3' end of growing polynucleotide chain DNA polymerase DNA s...

MMSC490 Exam 2 Questions with Complete Answers Which eukaryotic DNA polymerase replicates the lagging strand in the 3ʹ to 5ʹ direction? a. α b. γ c. δ d. None of the above; the lagging strand is replicated in the 5ʹ to 3ʹ direction. - Answer ️️ -d. None of the above; the lagging strand is replicated in the 5ʹ to 3ʹ direction. DNA polymerases can synthesize DNA: a. de novo, by catalyzing the polymerization of free dNTPs. b. by adding dNTPs to complementary dNTPs on a single-...