BIOS 390 (BIOS 390)

BIOS 390 Week 6 Exam Study Guide/Assignment

BIOS 390 WEEK 6: LAB: isolate Plasmid DNABIOS 390 BIOS . BIOS 390 WEEK 6: LAB: isolate Plasmid DNA Once a plasmid has been introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep of the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction diges...

BIOS 390 Week 6 Exam Study Guide/Assignment

Polymerase chain … (PCR) is a laboratory technique to increase, or make many copies of, a specific target region of DNA in vitro. The goal of PCR is to make enough of the target DNA region that it can … analyzed or used in some other way. For example, DNA amplified by PCR may … sent for sequencing, visualized by gel electrophoresis, or cloned into a plasmid for further experiments…. document continues

BIOS 390 Week 6 LAB: Isolate Plasmid DNA-Once a plasmid has … introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep of the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction digest on the purified DNA to determine whether the DNA insert in the ...

BIOS 390

BIOS390 – Molecular DiagnosticsWeek 1 (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: 1. The principle of segregation 2. The principle of independent assortment (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plants and then allowed the offspring to self-pollinate to produce an F2 generation. The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what relat...

ERIC KYLE Week 6 Exam Study Guide.

BIOS 390 – Molecular Biology Week 2 Quiz Study Guide Question Set 1 (TCO 3) DNA replication is said to be semiconservative because Each daughter DNA molecule consists of one strand from the parent DNA molecule and one new strand. (TCO 3) DNA synthesis occurs in The 5' to 3' direction. (TCO 3) Which of the following statements is correct? The growth and development or healing of wound in an organism require new cells. The new cells in organism come from division of...

BIOS 390 WEEK 3: LAB REPORT DNA cloning is the process of making multiple, identical copies of a certain piece of DNA. A gene or other DNA fragment of interest is first inserted into a circular piece of DNA called a plasmid. The insertion is done using enzymes that are able to “cut and paste” DNA, and then a molecule of recombinant DNA is produced. Next, the recombinant plasmid is introduced into bacteria. Bacteria carrying the plasmid are selected and grown up. As they reproduce, they repl...