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Exam (elaborations)
Biochem 4300 Exam Study Guide.
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Biochem 4300 Exam Study Guide. 
Flicks first law - AnswersA. Diffusion is driven by chemical potential 
B.the rate of diffusion is proportional to the concentration gradient 
C. Ficks first law law applies to steady state diffusion 
Ficks first law cab be used to determine D (diffusion coefficient) 
Diffusionis driven by chemical potential - AnswersMolecules move from a region of high 
concentration to a region of lower chemical po...
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Exam (elaborations)
Biochem 4300 Exam Questions With Verified Answers
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Biochem 4300 Exam Questions With Verified 
Answers 
Consider the reaction: aA + bB ® cC +dD. The reaction Gibbs energy can bewritten as .Q is 
called the______ and is equal to____ - Answersreaction quotient: ac^c x ad^d/ aA^Ax ab^b 
Delta r G < 0 for a reaction when the __________ predominate. The_________reaction is 
spontaneous; ____________formation dominates - Answersreactants , forward reaction, 
products 
Delta r G > 0...
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Biochem 4300 Exam 5 Detailed Questions With Verified Answers
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Biochem 4300 Exam 5 Detailed Questions 
With Verified Answers 
What is the bell analogy for NMR? - Answerseach atom has its own tumbling frequency 
What is the tumbling frequency of an atom affected by? - Answersits environment 
When does NMR occur? - Answerswhen the nucleus changes its spin status 
What is the nucleus changing its spin status driven by? - Answersabsorption of a quantum of 
energy 
What is the energy required for N...
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Exam (elaborations)
Biochem 4300 Exam 5 Questions With Verified Answers
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Biochem 4300 Exam 5 Questions With 
Verified Answers 
Chemical shift - Answersfrequency of nucleus relative to standard in magnetic field, measured 
in parts per million (ppm), position and # of chemical shifts diagnosis of STRUCTURE 
Tumbling frequency - Answerseach atom has it own, affected by environment and resonance 
(NMR spectroscopy) 
NMR active nuclei have... - Answershalf spin (I=1/2) quantum number 
for I=1/2 spin, the en...
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Exam (elaborations)
Biochem 4300 Final Exam Questions With Verified Answers
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Biochem 4300 Final Exam Questions With 
Verified Answers 
Spin status - Answersnuclear magnetic radiation (NMR) occurs when the nucleus changes its 
___ 
differences in T1 and T2 radiation - Answersin MRI, tissue contrast is determined by 
differences in ___ 
scalar coupling - Answersmediated through chemical bonds connecting two spins 
connectivity - Answersscalar coupling provides ___ information 
dipolar coupling - Answersmediat...
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Exam (elaborations)
Biochem 4300 Exam 3 Questions With Verified Answers
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Biochem 4300 Exam 3 Questions With 
Verified Answers 
Real time analysis - Answersrefers to the analysis of the reaction composition while the 
reaction is in progress 
uncompetitive inhibitor - Answersbinds to the enzyme-substrate complex, preventing the 
complex from releasing products 
competitive inhibitor - AnswersA substance that reduces the activity of an enzyme by entering 
the active site in place of the substrate whose st...
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Exam (elaborations)
Biochem 4300 Exam 3 Study Guide.
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Biochem 4300 Exam 3 Study Guide. 
Two ways to monitor the time dependence of a reaction - Answersreal time analysis 
fixed time assays 
real time analysis - Answersmonitor a reaction's composition while the reaction is in progress 
real time analysis allows you to see - Answersevery single substrate and intermediate 
throughout the reaction 
fixed time assays - Answersreaction runs a defined time amount and is then quenched and ...
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Exam (elaborations)
Biochem 4300 Exam 4 Questions With Verified Answers
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Biochem 4300 Exam 4 Questions With 
Verified Answers 
What is the mean free path? - Answersaverage distance traveled between collisions 
What is diffusion driven by? - Answerschemical potential 
What is flux (flow) defined as? - Answersamount of solute diffusing across a unit area per unit 
time 
What does Fick's first law state? - Answersthe rate of diffusion is proportional to concentration 
gradient 
What conditions do Fick...
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Exam (elaborations)
Biochem 4300 Exam 4 Questions With Verified Answers
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Biochem 4300 Exam 4 Questions With 
Verified Answers 
Fick's first law - AnswersThe diffusion flux is proportional to the concentration gradient. This 
relationship is used for steady-state diffusion situations. 
J(flux) = -D dc/dx 
D = diffusion coefficient 
diffusion coefficient (D) depends on... - Answersthe size of the molecule, viscosity of medium, 
and temperature 
determining D using Fick's first law - AnswersD= (n/[A*...
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