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Biochem 4300 Exam Study Guide.
Flicks first law - Answers✔A. Diffusion is driven by chemical potential
B.the rate of diffusion is proportional to the concentration gradient
C. Ficks first law law applies to steady state diffusion
Ficks first law cab be used to determine D (diffusion coefficient)
Diffusionis driven by chemical potential - Answers✔Molecules move from a region of high
concentration to a region of lower chemical potential
Ficks second law - Answers✔Second order partial differential equation. The solution is strongly
dependent on the system configuration
Ficks second law applies - Answers✔To non steady conditions eg concentration varies with time
K (dehydration) G - Answers✔203 kJ mol
Na (dehydration) G - Answers✔301 kJ mol
Electrophoresis type - Answers✔Agarose and acrylamide
Electrophoresis - Answers✔Electrophoretic mobility is a result of a constant drift speed
Mobility of a macromolecule depends on its net charge, size and shape
Protein electrophoresis with PI (4.8) - Answers✔Ph=3, positive charge , migrates towards -
electrode
Ph=4.8, neutral charge, no migration
Ph=7, negative charge, positive electrode
Sedimentation rate depends on - Answers✔Strength of the field on the masses and shapes of the
particles
It is very slow but can be accelerated by ultracentrifugation
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ALL RIGHTS RESERVED.
Biochem 4300 Exam Study Guide.
Flicks first law - Answers✔A. Diffusion is driven by chemical potential
B.the rate of diffusion is proportional to the concentration gradient
C. Ficks first law law applies to steady state diffusion
Ficks first law cab be used to determine D (diffusion coefficient)
Diffusionis driven by chemical potential - Answers✔Molecules move from a region of high
concentration to a region of lower chemical potential
Ficks second law - Answers✔Second order partial differential equation. The solution is strongly
dependent on the system configuration
Ficks second law applies - Answers✔To non steady conditions eg concentration varies with time
K (dehydration) G - Answers✔203 kJ mol
Na (dehydration) G - Answers✔301 kJ mol
Electrophoresis type - Answers✔Agarose and acrylamide
Electrophoresis - Answers✔Electrophoretic mobility is a result of a constant drift speed
Mobility of a macromolecule depends on its net charge, size and shape
Protein electrophoresis with PI (4.8) - Answers✔Ph=3, positive charge , migrates towards -
electrode
Ph=4.8, neutral charge, no migration
Ph=7, negative charge, positive electrode
Sedimentation rate depends on - Answers✔Strength of the field on the masses and shapes of the
particles
It is very slow but can be accelerated by ultracentrifugation
1
