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Quiz 2 Biochemistry Lab Chem 378|100% correct
- Exam (elaborations) • 3 pages • 2023
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Quiz 2 
Biochemistry Lab Chem 378 
Name:____________________ 
1. Below are restriction enzyme maps of two plasmids. Draw or described in numbers the 
expected bands of digestion in 1% agarose gel electrophoresis, relative to the 2 bands 
identified for you as control. 
2. Explain the different steps that are involved in a PCR experiment (include all the 
reagents used in the PCR mixture). 
5.5 kb 
BamHI 
3730 
PstI 
2650 
BamHI 
3050 
PstI 
1720 
PstI BamH1 PstI 
BamHI 
pCAG-EYFP 
4000 bp 
3000 ...
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