University Of Mississippi Medical Center

NPTE Practice Questions 2023 LATEST.

Glycogen is most abundant in - Answer Liver, heart (cardiac muscle cells), womb Staining reactions for glycogen: - Answer PAS (+), diastase (sensitive), Bauer-feulgen (+), Best carmine (+) Which two glycogen fixatives preserves the most glycogen? - Answer Bouins & Rossman's Other glycogen fixatives - Answer 80% alcohol, alcoholic formalin (37% formalin, 95% alcohol), acetic alcohol formalin, aqueous solution (formalin) This type of fixative should be avoided for glycogen fix...

This is a stain for acid mucopolysaccharides and sialomucins. - Answer Alcian blue This stains reacts with cations and is used to find calcium in tissue. - Answer Alizaran Red S This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - Answer Colloidal iron In this stain, tissue is first treated with alkali to open up binding sites through the release of hydrogen bonds. - Answer Congo Red This stain,combined with HCl and pa...

Cryptococcus neoformans - Answer a fungus that can be demonstrated with various stains for acidic mucins is the P.H. should be modified on the 4.6 stain - Answer ATPase stains done on a muscle biopsy at P.H.9.4,4.6 & 4.3.The slides for P.H.9.4 & 4.3 show good differentiation of the type 1 & 2,& the slide done at P.H.4.6 shows differentiation of type 1 & 2 fibers,it doesn't show differentiation of the type 2A and 2B fibers.This indicates that the hemochromatosis - Answer a patholo...

Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as additive to counter-act the shrinking effect of other fixatives. Formaldehyde - Answer Non coagulant and additive fixative that reacts with amino groups that link protein chains together....

Too much blade tilt causes this:? - Answer Chatter Chatter may be caused by? - Answer Cutting too rapidly Naphthol AS-D chloroacetate esterase stains are rare among enzyme techniques since it may be used on these type of sections? - Answer Formalin fixed paraffin

Cresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate. Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate. Bodain Method for Nerve Fibers - Answer Protargol (silver solution) with added COPPER destains connective tissue. Hydroquinone reduces, gold chloride, sodium thiosulfate follows.

Polarizing Microscope - Answer Microscope used to identify crystals, talc, silica, or urates. Birefringence - Answer Transmitting light unequally in different directions- Phase-Contrast Microscope - Answer Microscope used to examine unstained objects. Not routinely used in histopath labs.

Dehydrating Agents - Answer Alcohol, dioxane, acetone, tetrahydrofuran. Clearing Agents - Answer Xylene, toluene, benzene, acetone, chloroform, cedarwood oil, tetrahydrofuran, dioxane, limonene, aliphatic hydrocarbons. Universal Solvent - Answer Solvents that perform both the dehydration and clearing steps. Example Dioxane, Tetrahydrofuran.

Endoplasmic Reticulum - Answer H&E basophilic staining within cytoplasm is due to what cellular structure? Pink - Answer Cytoplasm will show up what color on an H&E? 20 - Answer Proteins are made up of combinations of how many amino acids? Carboxyl group, side group - Answer All amino acids have an amino group and what other groups? Amphoteric - Answer Proteins can be _______, meaning they can be an acid or a base. Peptide bonds - Answer Amino acids are linked by...