HTL Histotechnology: Special Stains questions with correct answers

This is a stain for acid mucopolysaccharides and sialomucins. - Answer Alcian blue This stains reacts with cations and is used to find calcium in tissue. - Answer Alizaran Red S This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - Answer Colloidal iron In this stain, tissue is first treated with alkali to open up binding sites through the release of hydrogen bonds. - Answer Congo Red This stain,combined with HCl and paraldehyde, stains elastic fibers and beta cells a deep purple. - Answer Gomori's Aldehyde Fuschin The first step of this silver stain is the oxidation of hexose sugars into aldehydes by potassium permanganate. - Answer Gomori's Stain for Reticulin Fibers In this stain, a plasma stain and a CT fibers stain are combined with phosphotungstic acid and acetic acid. Demonstrates collagen and muscle. - Answer Gomori's Trichrome This stain combines a PAS stain, Weigert's hematoxylin, and a selective collagen stain combining picric acid and methyl blue. - Answer Lillie's Allochrome