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Bio 260 Lab Final Scc Questions And Verified Answers

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BIO 260 LAB FINAL SCC QUESTIONS AND VERIFIED ANSWERS

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Anaerobic respiration involves the reduction of an inorganic molecule other than
oxygen. Nitrate reduction is one such exampe. Many _______ bacteria can reduce nitrate
to nitrite.


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Gram-negative




Describe the theory behind the EnteroPluri Test.


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, It's a multiple test system designed to identify enteric bacteria based on:
glucose, adonitrol, lactose, arabinose, sorbitol, and dulcitol fermentation;
lysine and ornithine decarboxylation; sulfur reduction; indole production;
acetoin production from glucose fermentation; phenylalanine deamination;
urea hydrolysis; and citrate utilization.




Define antibodies and antigens?


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-Antibodies: Proteins that recognize antigens and bind tightly to them

-Antigens: Harmful bacteria/protein




How did the bacterial grow when they were covered by cardboard mask during UV
exposure?


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The heavy growth on the plates covered by the cardboard during exposure
shows that UV radiation was successfully blocked by the cardboard
because the rays weren't able to reach many bacteria cells and kill them




What is the purpose in having a control when using differential and selective media.


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Serves as a comparison for growth quality.
Control plates in microbiology use specified control strains of bacterial that

, have a known result in any given test.




Give 2 examples of human pathogens that form endospores?


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Clostridium difficile
Clostridium tetani




Give an example of a basic dye?


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Crystal violet




What does a simple stain look like?


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Describe obligate aerobe, and obligate anaerobe?

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Obligate aerobe: Require oxygen to live
Obligate anaerobe: even a small amount of oxygen is lethal
In picture #1 is obligate aerobe and #2 is an obligate
anaerobe.




What function does zinc perform in the nitrate test?


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A chemical reducing agent




Name the selective agent and the only fermentable carbohydrate in MacConkey
Agar?


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-Selective agent
Bile salts and crystal violet inhibit growth of gram+

-Fermentable carbohydrate
Lactose




List 5 features of a colony that may be described when reporting colonial
morphology?


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